This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
GAPDH Antibody
catalog :
AB2302
quantity :
100 μg
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
human, mouse, dogs
application :
western blot
citations: 31
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:5000; loading ...; fig 3d
Lechertier T, Reynolds L, Kim H, Pedrosa A, Gómez Escudero J, Muñoz Félix J, et al. Pericyte FAK negatively regulates Gas6/Axl signalling to suppress tumour angiogenesis and tumour growth. Nat Commun. 2020;11:2810 pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 1b
Chiang A, Fowler S, Savjani R, Hilsenbeck S, Wallace C, Cirrito J, et al. Combination anti-Aβ treatment maximizes cognitive recovery and rebalances mTOR signaling in APP mice. J Exp Med. 2018;215:1349-1364 pubmed publisher
  • western blot; human; loading ...; fig 6a
Yuan W, Guo Y, Li X, Deng M, Shen Z, Bo C, et al. MicroRNA-126 inhibits colon cancer cell proliferation and invasion by targeting the chemokine (C-X-C motif) receptor 4 and Ras homolog gene family, member A, signaling pathway. Oncotarget. 2016;7:60230-60244 pubmed publisher
  • western blot; mouse; 1:40,000; fig 1
Auderset L, Cullen C, Young K. Low Density Lipoprotein-Receptor Related Protein 1 Is Differentially Expressed by Neuronal and Glial Populations in the Developing and Mature Mouse Central Nervous System. PLoS ONE. 2016;11:e0155878 pubmed publisher
  • western blot; mouse; 1:10,000; fig 4
Yetman M, Fowler S, Jankowsky J. Humanized Tau Mice with Regionalized Amyloid Exhibit Behavioral Deficits but No Pathological Interaction. PLoS ONE. 2016;11:e0153724 pubmed publisher
  • western blot; human; fig s8
Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
  • western blot; mouse; 1:5000; fig 6
Wang W, Jossin Y, Chai G, Lien W, Tissir F, Goffinet A. Feedback regulation of apical progenitor fate by immature neurons through Wnt7-Celsr3-Fzd3 signalling. Nat Commun. 2016;7:10936 pubmed publisher
  • western blot; human; 1:5000; fig s1
Kenific C, Stehbens S, Goldsmith J, Leidal A, Faure N, Ye J, et al. NBR1 enables autophagy-dependent focal adhesion turnover. J Cell Biol. 2016;212:577-90 pubmed publisher
  • western blot; mouse; fig s8
Ahuja A, Jodkowska K, Teloni F, Bizard A, Zellweger R, Herrador R, et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016;7:10660 pubmed publisher
Brekk O, Korecka J, Crapart C, Huebecker M, MacBain Z, Rosenthal S, et al. Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity. Acta Neuropathol Commun. 2020;8:127 pubmed publisher
Potjewyd F, Turner A, Beri J, Rectenwald J, Norris Drouin J, Cholensky S, et al. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader. Cell Chem Biol. 2019;: pubmed publisher
Marquié M, Agüero C, Amaral A, Villarejo Galende A, Ramanan P, Chong M, et al. [18F]-AV-1451 binding profile in chronic traumatic encephalopathy: a postmortem case series. Acta Neuropathol Commun. 2019;7:164 pubmed publisher
Uzquiano A, Cifuentes Diaz C, Jabali A, Romero D, Houllier A, Dingli F, et al. Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia. Cell Rep. 2019;28:1596-1611.e10 pubmed publisher
Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux M, et al. Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors. Cell Rep. 2018;25:130-145.e5 pubmed publisher
Zhao H, Wang L, Chen L, Zhang J, Sun W, Salvi R, et al. Temporary conductive hearing loss in early life impairs spatial memory of rats in adulthood. Brain Behav. 2018;8:e01004 pubmed publisher
Hallett P, Huebecker M, Brekk O, Moloney E, Rocha E, Priestman D, et al. Glycosphingolipid levels and glucocerebrosidase activity are altered in normal aging of the mouse brain. Neurobiol Aging. 2018;67:189-200 pubmed publisher
Liu P, Feng T, Zuo X, Wang X, Luo J, Li N, et al. A novel SIRT1 activator E6155 improves insulin sensitivity in type 2 diabetic KKAy mice. Biochem Biophys Res Commun. 2018;498:633-639 pubmed publisher
Sutton L, Orlandi C, Song C, Oh W, Muntean B, Xie K, et al. Orphan receptor GPR158 controls stress-induced depression. elife. 2018;7: pubmed publisher
Weng Y, Wang X, An R, Cassin J, Vissers C, Liu Y, et al. Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. Neuron. 2018;97:313-325.e6 pubmed publisher
Rodgers J, Samal E, Mohapatra S, PANGULURI S. Hyperoxia-induced cardiotoxicity and ventricular remodeling in type-II diabetes mice. Heart Vessels. 2018;33:561-572 pubmed publisher
Barter M, Gomez R, Hyatt S, Cheung K, Skelton A, Xu Y, et al. The long non-coding RNA ROCR contributes to SOX9 expression and chondrogenic differentiation of human mesenchymal stem cells. Development. 2017;144:4510-4521 pubmed publisher
Basu R, Duan X, Taylor M, Martin E, Muralidhar S, Wang Y, et al. Heterophilic Type II Cadherins Are Required for High-Magnitude Synaptic Potentiation in the Hippocampus. Neuron. 2017;96:160-176.e8 pubmed publisher
Satterfield L, Shuck R, Kurenbekova L, Allen Rhoades W, Edwards D, Huang S, et al. miR-130b directly targets ARHGAP1 to drive activation of a metastatic CDC42-PAK1-AP1 positive feedback loop in Ewing sarcoma. Int J Cancer. 2017;141:2062-2075 pubmed publisher
Bu Q, Wang A, Hamzah H, Waldman A, Jiang K, Dong Q, et al. CREB Signaling Is Involved in Rett Syndrome Pathogenesis. J Neurosci. 2017;37:3671-3685 pubmed publisher
Wang Y, Fehlhaber K, Sarria I, Cao Y, Ingram N, Guerrero Given D, et al. The Auxiliary Calcium Channel Subunit α2δ4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors. Neuron. 2017;93:1359-1374.e6 pubmed publisher
Ingrassia R, Memo M, Garavaglia B. Ferrous Iron Up-regulation in Fibroblasts of Patients with Beta Propeller Protein-Associated Neurodegeneration (BPAN). Front Genet. 2017;8:18 pubmed publisher
Pantoja Melendez C, Miranda Duarte A, Roque Ramírez B, Zenteno J. Epidemiological and Molecular Characterization of a Mexican Population Isolate with High Prevalence of Limb-Girdle Muscular Dystrophy Type 2A Due to a Novel Calpain-3 Mutation. PLoS ONE. 2017;12:e0170280 pubmed publisher
Damiani D, Goffinet A, ALBERTS A, Tissir F. Lack of Diaph3 relaxes the spindle checkpoint causing the loss of neural progenitors. Nat Commun. 2016;7:13509 pubmed publisher
Renner G, Noulet F, Mercier M, Choulier L, Etienne Selloum N, Gies J, et al. Expression/activation of ?5?1 integrin is linked to the ?-catenin signaling pathway to drive migration in glioma cells. Oncotarget. 2016;7:62194-62207 pubmed publisher
Beltran H, Prandi D, Mosquera J, Benelli M, Puca L, Cyrta J, et al. Divergent clonal evolution of castration-resistant neuroendocrine prostate cancer. Nat Med. 2016;22:298-305 pubmed publisher
Kruiswijk F, Hasenfuss S, Sivapatham R, Baar M, Putavet D, Naipal K, et al. Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling. Oncogene. 2016;35:2166-77 pubmed publisher
product information
Catalog Number :
AB2302
Subcategory :
Metabolism
Product Name :
Anti-GAPDH Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
P04406
Host Name :
Chicken
Antigen :
GAPDH
Conjugate :
Affinity Purified
Product Description :
Anti-GAPDH Antibody
Cross Reactivity :
Human;Mouse;Rat;Horse;Canine
Background :
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
ALT Names :
glyceraldehyde 3-phosphate dehydrogenase;aging-associated gene 9 protein;glyceraldehyde-3-phosphate dehydrogenase
Immunogen :
KLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.
Specificity :
This antibody recognizes GAPDH.
Package Size :
100 μg
Uses :
Western Blotting
Storage :
Stable for 1 year at 2-8°C from date of receipt
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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