Doublecortin Antibody | EMD Millipore AB2253 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Doublecortin Antibody

catalog :

AB2253

quantity :

50 µL

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:400; loading ...; fig s1b	Mayerl S, Heuer H, Ffrench Constant C. Hippocampal Neurogenesis Requires Cell-Autonomous Thyroid Hormone Signaling. Stem Cell Reports. 2020;14:845-860 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 5a, b	Shibahara T, Ago T, Nakamura K, Tachibana M, Yoshikawa Y, Komori M, et al. Pericyte-Mediated Tissue Repair through PDGFRβ Promotes Peri-Infarct Astrogliosis, Oligodendrogenesis, and Functional Recovery after Acute Ischemic Stroke. Eneuro. 2020;7: pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 2d	Kjell J, Fischer Sternjak J, Thompson A, Friess C, Sticco M, Salinas F, et al. Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell. 2020;26:277-293.e8 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 2a	Anstötz M, Maccaferri G. A Toolbox of Criteria for Distinguishing Cajal-Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus. Eneuro. 2020;7: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3b	Robison L, Albert N, Camargo L, Anderson B, Salinero A, Riccio D, et al. High-Fat Diet-Induced Obesity Causes Sex-Specific Deficits in Adult Hippocampal Neurogenesis in Mice. Eneuro. 2020;7: pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 4a	Upadhyay A, Hosseinibarkooie S, Schneider S, Kaczmarek A, Torres Benito L, Mendoza Ferreira N, et al. Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological. Front Mol Neurosci. 2019;12:19 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig 1b	Kalamakis G, Brune D, Ravichandran S, Bolz J, Fan W, Ziebell F, et al. Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Cell. 2019;: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5a	Hill J, Zuluaga Ramirez V, Gajghate S, Winfield M, Sriram U, Rom S, et al. Activation of GPR55 induces neuroprotection of hippocampal neurogenesis and immune responses of neural stem cells following chronic, systemic inflammation. Brain Behav Immun. 2019;76:165-181 pubmed publisher
immunohistochemistry; mouse; 1:400; loading ...; fig 4a	Qiu C, Liu Z, Hou K, Liu S, Hu Y, Zhang L, et al. Wip1 knockout inhibits neurogenesis by affecting the Wnt/β-catenin signaling pathway in focal cerebral ischemia in mice. Exp Neurol. 2018;309:44-53 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 4a	Paul A, Chaker Z, Doetsch F. Hypothalamic regulation of regionally distinct adult neural stem cells and neurogenesis. Science. 2017;356:1383-1386 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1e	Jongbloets B, Lemstra S, Schellino R, Broekhoven M, Parkash J, Hellemons A, et al. Stage-specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors. Nat Commun. 2017;8:14666 pubmed publisher
immunocytochemistry; human; fig 3a	Bershteyn M, Nowakowski T, Pollen A, Di Lullo E, Nene A, Wynshaw Boris A, et al. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia. Cell Stem Cell. 2017;20:435-449.e4 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 1a	Achuta V, Grym H, Putkonen N, Louhivuori V, Kärkkäinen V, Koistinaho J, et al. Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome. Dev Neurobiol. 2017;77:438-453 pubmed publisher
	Liu J, Matarin M, REEVES C, McEvoy A, Miserocchi A, Thompson P, et al. Doublecortin-expressing cell types in temporal lobe epilepsy. Acta Neuropathol Commun. 2018;6:60 pubmed publisher
	Hill J, Zuluaga Ramirez V, Gajghate S, Winfield M, Persidsky Y. Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis. Br J Pharmacol. 2018;175:3407-3421 pubmed publisher
	Jung M, Häberle B, Tschaikowsky T, Wittmann M, Balta E, Stadler V, et al. Analysis of the expression pattern of the schizophrenia-risk and intellectual disability gene TCF4 in the developing and adult brain suggests a role in development and plasticity of cortical and hippocampal neurons. Mol Autism. 2018;9:20 pubmed publisher
	Hendricks W, Chen Y, Bensen A, Westbrook G, Schnell E. Short-Term Depression of Sprouted Mossy Fiber Synapses from Adult-Born Granule Cells. J Neurosci. 2017;37:5722-5735 pubmed publisher
	Brulet R, Zhu J, Aktar M, Hsieh J, Cho K. Mice with conditional NeuroD1 knockout display reduced aberrant hippocampal neurogenesis but no change in epileptic seizures. Exp Neurol. 2017;293:190-198 pubmed publisher
	Falk S, Bugeon S, Ninkovic J, Pilz G, Postiglione M, Cremer H, et al. Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding. Neuron. 2017;93:777-791.e3 pubmed publisher
	Beckervordersandforth R, Ebert B, Schäffner I, Moss J, Fiebig C, Shin J, et al. Role of Mitochondrial Metabolism in the Control of Early Lineage Progression and Aging Phenotypes in Adult Hippocampal Neurogenesis. Neuron. 2017;93:560-573.e6 pubmed publisher
	Soto Rodríguez S, Lopez Armas G, Luquin S, Ramos Zúñiga R, Jauregui Huerta F, Gonzalez Perez O, et al. Rapid Eye Movement Sleep Deprivation Produces Long-Term Detrimental Effects in Spatial Memory and Modifies the Cellular Composition of the Subgranular Zone. Front Cell Neurosci. 2016;10:132 pubmed publisher
	Daviaud N, Chen K, Huang Y, Friedel R, Zou H. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol. 2016;76:882-99 pubmed publisher
	Kotterman M, Vazin T, Schaffer D. Enhanced selective gene delivery to neural stem cells in vivo by an adeno-associated viral variant. Development. 2015;142:1885-92 pubmed publisher
	Paez Gonzalez P, Asrican B, Rodriguez E, Kuo C. Identification of distinct ChAT? neurons and activity-dependent control of postnatal SVZ neurogenesis. Nat Neurosci. 2014;17:934-42 pubmed publisher

product information

Catalog Number :

AB2253

Subcategory :

Neuroscience

Product Name :

Anti-Doublecortin Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O43602

Host Name :

Guinea Pig

Antigen :

Doublecortin

Conjugate :

Serum

Product Description :

Anti-Doublecortin Antibody

Cross Reactivity :

Rat;Mouse

Background :

Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis.

ALT Names :

doublecortex; lissencephaly, X-linked (doublecortin)

Immunogen :

Recombinant Peptide.

Specificity :

Cat. # AB2253 recognizes the C-Terminus of Doublecortin.

Package Size :

50 µL

Uses :

Western Blotting;Immunohistochemistry;Immunocytochemistry

Storage :

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.