Fibronectin Antibody | EMD Millipore AB2047 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Fibronectin Antibody

catalog :

AB2047

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, radioimmunoassay, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 5 ug/ml; loading ...; fig 3	Ertsås H, Nolan G, Labarge M, Lorens J. Microsphere cytometry to interrogate microenvironment-dependent cell signaling. Integr Biol (Camb). 2017;9:123-134 pubmed publisher
	Roman W, Martins J, Gomes E. Local Arrangement of Fibronectin by Myofibroblasts Governs Peripheral Nuclear Positioning in Muscle Cells. Dev Cell. 2018;46:102-111.e6 pubmed publisher

product information

Catalog Number :

AB2047

Subcategory :

Cell Structure

Product Name :

Anti-Fibronectin Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P02751

Host Name :

Rabbit

Antigen :

Fibronectin

Conjugate :

Purified

Product Description :

Anti-Fibronectin Antibody

Cross Reactivity :

Bovine

Background :

Fibronectin (FN) is an extracellular adhesion molecule and it is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}.

Immunogen :

Fibronectin extracted and purified from bovine plasma.

Specificity :

Antibody reacts with bovine fibronectin, and demonstrates cross-reactivity of less than 0.1% with bovine collagens types I, III, IV by RIA at 1:10,000 dilution.

Package Size :

100 µg

Uses :

ELISA;Immunofluorescence;Radioimmunoassay;Western Blotting;Immunohistochemistry (Paraffin)

Storage :

Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.