Integrin alphaV Antibody, CT, Intracellular | EMD Millipore AB1930 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin alphaV Antibody, CT, Intracellular

catalog :

AB1930

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dogs, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, radioimmunoassay

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; dogs; loading ...; fig 2d	Zhang K, MyllymÃ¤ki S, Gao P, Devarajan R, KytÃ¶lÃ¤ V, Nykter M, et al. Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce anoikis resistance and synergizes with Î±V-Class integrins to promote EMT. Oncogene. 2017;36:5681-5694 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s2a	Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 8e	Kishi T, Mayanagi T, Iwabuchi S, Akasaka T, Sobue K. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget. 2016;7:72113-72130 pubmed publisher
western blot; mouse; 1:1000; fig 2	Chen Z, Ding X, Jin S, Pitt B, Zhang L, Billiar T, et al. WISP1-?v?3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury. Sci Rep. 2016;6:28841 pubmed publisher
immunocytochemistry; human; 1:500; fig 2	Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Arima M, et al. Tenascin-C promotes angiogenesis in fibrovascular membranes in eyes with proliferative diabetic retinopathy. Mol Vis. 2016;22:436-45 pubmed

product information

Catalog Number :

AB1930

Subcategory :

Cell Structure

Product Name :

Anti-Integrin alphaV Antibody, CT, Intracellular

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P06756

Host Name :

Rabbit

Antigen :

Integrin alphaV

Conjugate :

Serum

Product Description :

Anti-Integrin alphaV Antibody, CT, Intracellular

Cross Reactivity :

Chicken;Horse;Goat;Human;Mouse;Hamster;Rat;Pig;Sheep

Background :

The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.

ALT Names :

CD51

Immunogen :

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alphaV integrin subunit (SwissProt Accession P06756, amino acids 1022-1034).

Specificity :

Integrin alphaV. Specificity determined by immunoprecipitation from a detergent extract of 35S methonine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, or alpha6 integrin subunits was detected. The mature alphaV protein (150 kDa) is composed of a disulfide bonded heavy (125 kDa) and a light (25 kDa) chain derived from a proteolytically cleaved precursor (Suzuki, 1987). The epitope recognized by the antibody is located on the COOH terminal portion of the light chain. By western blot under reducing conditions the antibody detects two major bands at approximately 25 and 27 kDa corresponding to the light chain. The slight differences in sizes of the light chains is thought to be caused by proteolytic cleavage at two alternative sites on the precursor. Similar sites have been reported for alpha6, alpha3, and alphalIbeta (Hogervorst, 1991).

Package Size :

100 µL

Uses :

ELISA;Immunohistochemistry;Immunoprecipitation;Radioimmunoassay;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.