Integrin α5 Antibody, CT, Intracellular | EMD Millipore AB1928 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin α5 Antibody, CT, Intracellular

catalog :

AB1928

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, radioimmunoassay

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; fig 6	Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
immunocytochemistry; rat; 1:500; fig 1	Heintz T, Eva R, Fawcett J. Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. PLoS ONE. 2016;11:e0158558 pubmed publisher
western blot; human; fig 3g	Khan S, Sikander M, Ebeling M, Ganju A, Kumari S, Yallapu M, et al. MUC13 interaction with receptor tyrosine kinase HER2 drives pancreatic ductal adenocarcinoma progression. Oncogene. 2017;36:491-500 pubmed publisher
	Gagné D, Benoit Y, Groulx J, Vachon P, Beaulieu J. ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process. BMC Mol Cell Biol. 2020;21:14 pubmed publisher
	Kawamura E, Hamilton G, Miskiewicz E, MacPhee D. Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion. BMC Dev Biol. 2018;18:19 pubmed publisher
	Nair M, Romero J, Mahtabfar A, Meleis A, Foty R, Corbett S. Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo. Int J Mol Sci. 2018;19: pubmed publisher
	Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T. Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons. Sci Rep. 2017;7:42111 pubmed publisher
	Sung B, Weaver A. Exosome secretion promotes chemotaxis of cancer cells. Cell Adh Migr. 2017;11:187-195 pubmed publisher
	Strachan L, Condic M. Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility. J Cell Biol. 2004;167:545-54 pubmed

product information

Catalog Number :

AB1928

Subcategory :

Cell Structure

Product Name :

Anti-Integrin α5 Antibody, CT, Intracellular

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P08648

Host Name :

Rabbit

Antigen :

Integrin α5

Conjugate :

Serum

Product Description :

Anti-Integrin α5 Antibody, CT, Intracellular

Cross Reactivity :

Chicken;Human;Mouse;Hamster;Rat

Background :

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

ALT Names :

CD49e;Fibronectin Receptor alpha Subunit

Immunogen :

Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.

Specificity :

Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.

Package Size :

100 µL

Uses :

ELISA;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunoprecipitation;Radioimmunoassay;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.