Tryptophan Hydroxylase Antibody | EMD Millipore AB1541 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tryptophan Hydroxylase Antibody

catalog :

AB1541

quantity :

100 µL

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunohistochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig 3a	Moriya S, Yamashita A, Masukawa D, Kambe Y, Sakaguchi J, Setoyama H, et al. Involvement of supralemniscal nucleus (B9) 5-HT neuronal system in nociceptive processing: a fiber photometry study. Mol Brain. 2020;13:14 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; loading ...; fig 3c	Veres A, Faust A, Bushnell H, Engquist E, Kenty J, Harb G, et al. Charting cellular identity during human in vitro β-cell differentiation. Nature. 2019;569:368-373 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 4	Dengler Crish C, Smith M, Wilson G. Early Evidence of Low Bone Density and Decreased Serotonergic Synthesis in the Dorsal Raphe of a Tauopathy Model of Alzheimer's Disease. J Alzheimers Dis. 2017;55:1605-1619 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:2000	Landry J, Hawkins C, Lee A, CotÃ© A, Balaban E, Pompeiano M. Chick embryos have the same pattern of hypoxic lower-brain activation as fetal mammals. Dev Neurobiol. 2016;76:64-74 pubmed publisher
	Nagai Y, Takayama K, Nishitani N, Andoh C, Koda M, Shirakawa H, et al. The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion. Int J Mol Sci. 2020;21: pubmed publisher
	Soiza Reilly M, Meye F, Olusakin J, Telley L, Petit E, Chen X, et al. SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior. Mol Psychiatry. 2019;24:726-745 pubmed publisher
	Babb J, Linnros S, Commons K. Evidence for intact 5-HT1A receptor-mediated feedback inhibition following sustained antidepressant treatment in a rat model of depression. Neuropharmacology. 2018;141:139-147 pubmed publisher
	Vahid Ansari F, Daigle M, Manzini M, Tanaka K, Hen R, Geddes S, et al. Abrogated Freud-1/Cc2d1a Repression of 5-HT1A Autoreceptors Induces Fluoxetine-Resistant Anxiety/Depression-Like Behavior. J Neurosci. 2017;37:11967-11978 pubmed publisher
	Panzini C, Ehlinger D, Alchahin A, Guo Y, Commons K. 16p11.2 deletion syndrome mice perseverate with active coping response to acute stress - rescue by blocking 5-HT2A receptors. J Neurochem. 2017;143:708-721 pubmed publisher
	Stemme T, Stern M, Bicker G. Serotonin-containing neurons in basal insects: In search of ground patterns among tetraconata. J Comp Neurol. 2017;525:79-115 pubmed publisher

product information

Catalog Number :

AB1541

Subcategory :

Neuroscience

Product Name :

Anti-Tryptophan Hydroxylase Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P17752

Host Name :

Sheep

Antigen :

Tryptophan Hydroxylase

Conjugate :

Affinity Purified

Product Description :

Anti-Tryptophan Hydroxylase Antibody

Cross Reactivity :

Human;Rat

Background :

Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.

ALT Names :

Tryptophan 5-monooxygenase 1;indoleacetic acid-5-hydroxylase;tryptophan 5-hydroxylase;tryptophan 5-monooxygenase;tryptophan hydroxylase (tryptophan 5-monooxygenase);tryptophan hydroxylase 1

Immunogen :

Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Specificity :

Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.

Package Size :

100 µL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.