Glutamate Decarboxylase 65 & 67 Antibody | EMD Millipore AB1511 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Glutamate Decarboxylase 65 & 67 Antibody

catalog :

AB1511

quantity :

50 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig s6a	Chen B, Xu C, Wang Y, Lin W, Wang Y, Chen L, et al. A disinhibitory nigra-parafascicular pathway amplifies seizure in temporal lobe epilepsy. Nat Commun. 2020;11:923 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig 1f	Lorenzo L, Godin A, Ferrini F, Bachand K, Plasencia Fernandez I, Labrecque S, et al. Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain. Nat Commun. 2020;11:869 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 1d western blot; human; 1:200; loading ...; fig 1b	Vogel S, Schäfer C, Hess S, Folz Donahue K, Nelles M, Minassian A, et al. The in vivo timeline of differentiation of engrafted human neural progenitor cells. Stem Cell Res. 2019;37:101429 pubmed publisher
immunocytochemistry; human; 1:200; fig s23	Sousa A, Zhu Y, Raghanti M, Kitchen R, Onorati M, Tebbenkamp A, et al. Molecular and cellular reorganization of neural circuits in the human lineage. Science. 2017;358:1027-1032 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:3000; loading ...; fig 3a	Hayashi K, Furuya A, Sakamaki Y, Akagi T, Shinoda Y, Sadakata T, et al. The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination. PLoS ONE. 2017;12:e0173175 pubmed publisher
immunocytochemistry; human; 1:500; loading ...; fig st4	Qi Y, Zhang X, Renier N, Wu Z, Atkin T, Sun Z, et al. Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells. Nat Biotechnol. 2017;35:154-163 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; tbl 1	Kordower J, Goetz C, Chu Y, Halliday G, Nicholson D, Musial T, et al. Robust graft survival and normalized dopaminergic innervation do not obligate recovery in a Parkinson disease patient. Ann Neurol. 2017;81:46-57 pubmed publisher
immunocytochemistry; human; 1:2400; loading ...; tbl s4	Hansen S, Stummann T, Borland H, Hasholt L, Tumer Z, Nielsen J, et al. Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3. Stem Cell Res. 2016;17:306-317 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig s4d	Tillberg P, Chen F, Piatkevich K, Zhao Y, Yu C, English B, et al. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol. 2016;34:987-92 pubmed publisher
western blot; rat; fig 3g	Fuente Martín E, García Cáceres C, Argente Arizón P, Diaz F, Granado M, Freire Regatillo A, et al. Ghrelin Regulates Glucose and Glutamate Transporters in Hypothalamic Astrocytes. Sci Rep. 2016;6:23673 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 3e	Ho S, Hartley B, TCW J, Beaumont M, Stafford K, Slesinger P, et al. Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells. Methods. 2016;101:113-24 pubmed publisher
immunocytochemistry; human; 1:1000	Ahn S, Kim T, Kim K, Chung S. Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells. Methods. 2016;101:103-12 pubmed publisher
	Docampo Seara A, Pereira Guldrís S, Sánchez Farías N, Mazan S, Rodriguez M, Candal E. Characterization of neurogenic niches in the telencephalon of juvenile and adult sharks. Brain Struct Funct. 2020;225:817-839 pubmed publisher
	Korecki A, Hickmott J, Lam S, Dreolini L, Mathelier A, Baker O, et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics. 2019;211:1155-1177 pubmed publisher
	Yazdan Shahmorad A, Silversmith D, Kharazia V, Sabes P. Targeted cortical reorganization using optogenetics in non-human primates. elife. 2018;7: pubmed publisher
	Wang C, Najm R, Xu Q, Jeong D, Walker D, Balestra M, et al. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. Nat Med. 2018;24:647-657 pubmed publisher
	Ray T, Roy S, Kozlowski C, Wang J, Cafaro J, Hulbert S, et al. Formation of retinal direction-selective circuitry initiated by starburst amacrine cell homotypic contact. elife. 2018;7: pubmed publisher
	Carreras I, Aytan N, Mellott T, Choi J, Lehar M, Crabtree L, et al. Anxiety, neuroinflammation, cholinergic and GABAergic abnormalities are early markers of Gulf War illness in a mouse model of the disease. Brain Res. 2018;1681:34-43 pubmed publisher
	Li Y, Chen Z, Gao Y, Pan G, Zheng H, Zhang Y, et al. Synaptic Adhesion Molecule Pcdh-γC5 Mediates Synaptic Dysfunction in Alzheimer's Disease. J Neurosci. 2017;37:9259-9268 pubmed publisher
	Petri R, Pircs K, Jönsson M, Akerblom M, Brattås P, Klussendorf T, et al. let-7 regulates radial migration of new-born neurons through positive regulation of autophagy. EMBO J. 2017;36:1379-1391 pubmed publisher
	Park K, Randazzo G, Jones K, Brzezinski J. Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina. Invest Ophthalmol Vis Sci. 2017;58:1137-1150 pubmed publisher
	Bonin R, Wang F, Desrochers Couture M, Ga Secka A, Boulanger M, Côté D, et al. Epidural optogenetics for controlled analgesia. Mol Pain. 2016;12: pubmed publisher
	MendonÃ§a M, Soares E, StÃ¡vale L, Kalapothakis E, Cruz HÃ¶fling M. Vascular endothelial growth factor increases during blood-brain barrier-enhanced permeability caused by Phoneutria nigriventer spider venom. Biomed Res Int. 2014;2014:721968 pubmed publisher

product information

Catalog Number :

AB1511

Subcategory :

Neuroscience

Product Name :

Anti-Glutamate Decarboxylase 65 & 67 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q05329

Host Name :

Rabbit

Antigen :

Glutamate Decarboxylase 65 & 67

Conjugate :

Purified

Product Description :

Anti-Glutamate Decarboxylase 65 & 67 Antibody

Cross Reactivity :

Human;Rat;Mouse;Feline

Background :

Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.

ALT Names :

65 kDa glutamic acid decarboxylase;Glutamate decarboxylase 65 kDa isoform;Glutamate decarboxylase-2 (pancreas);glutamate decarboxylase 2;glutamate decarboxylase 2 (pancreatic islets and brain,65kD);glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

Immunogen :

A synthetic peptide with the amino acid sequence [C]DFLIEEIERLGQDL from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys] + 572-585) (1).

Specificity :

Glutamate Decarboxylase (GAD65 and GAD67). Western blot reveals a doublet at approximately 65/68 kDa. Immunohistochemical staining can be abolished by preincubation with 1-10 μg peptide (Catalog Number AG252) per mL of diluted antibody.

Package Size :

50 µL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.