Choline Acetyltransferase (ChAT) Antibody | EMD Millipore AB143 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Choline Acetyltransferase (ChAT) Antibody

catalog :

AB143

quantity :

50 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:600; loading ...; fig 4e	Crevier Sorbo G, Rymar V, Crevier Sorbo R, Sadikot A. Thalamostriatal degeneration contributes to dystonia and cholinergic interneuron dysfunction in a mouse model of Huntington's disease. Acta Neuropathol Commun. 2020;8:14 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:300; fig e1i	Nonomura K, Woo S, Chang R, Gillich A, Qiu Z, Francisco A, et al. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature. 2017;541:176-181 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 2	Lee N, Rydyznski C, Rasch M, Trinh D, MacLennan A. Adult ciliary neurotrophic factor receptors help maintain facial motor neuron choline acetyltransferase expression in vivo following nerve crush. J Comp Neurol. 2017;525:1206-1215 pubmed publisher
immunocytochemistry; mouse; 1:5000; loading ...; fig 1c	Hamamoto M, Kiyokage E, Sohn J, Hioki H, Harada T, Toida K. Structural basis for cholinergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol. 2017;525:574-591 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 3	Senzacqua M, Severi I, Perugini J, Acciarini S, Cinti S, Giordano A. Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex. Front Neurosci. 2016;10:289 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig s1	Nakashima H, Ohkawara B, Ishigaki S, Fukudome T, Ito K, Tsushima M, et al. R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5. Sci Rep. 2016;6:28512 pubmed publisher
immunocytochemistry; human; 1:200; fig 3	Allodi I, Comley L, Nichterwitz S, Nizzardo M, Simone C, Benitez J, et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep. 2016;6:25960 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...	Pabst M, Braganza O, Dannenberg H, Hu W, Pothmann L, Rosen J, et al. Astrocyte Intermediaries of Septal Cholinergic Modulation in the Hippocampus. Neuron. 2016;90:853-65 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig 3	Liput D, Lu V, Davis M, Puhl H, Ikeda S. Rem2, a member of the RGK family of small GTPases, is enriched in nuclei of the basal ganglia. Sci Rep. 2016;6:25137 pubmed publisher
immunohistochemistry; rhesus macaque; 1:500	Mueller A, Davis A, Sovich S, Carlson S, Robinson F. Distribution of N-Acetylgalactosamine-Positive Perineuronal Nets in the Macaque Brain: Anatomy and Implications. Neural Plast. 2016;2016:6021428 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 2	Ahn M, Kalume F, Pitstick R, Oehler A, Carlson G, Dearmond S. Brain Aggregates: An Effective In Vitro Cell Culture System Modeling Neurodegenerative Diseases. J Neuropathol Exp Neurol. 2016;75:256-62 pubmed publisher
	Fan W, Li X, Yao H, Deng J, Liu H, Cui Z, et al. Neural differentiation and synaptogenesis in retinal development. Neural Regen Res. 2016;11:312-8 pubmed publisher
	Yang C, McKenna J, Zant J, Winston S, Basheer R, Brown R. Cholinergic neurons excite cortically projecting basal forebrain GABAergic neurons. J Neurosci. 2014;34:2832-44 pubmed publisher
	Röhn T, Ralvenius W, Paul J, Borter P, Hernandez M, Witschi R, et al. A virus-like particle-based anti-nerve growth factor vaccine reduces inflammatory hyperalgesia: potential long-term therapy for chronic pain. J Immunol. 2011;186:1769-80 pubmed publisher

product information

Catalog Number :

AB143

Subcategory :

Neuroscience

Product Name :

Anti-Choline Acetyltransferase (ChAT) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P28329

Host Name :

Rabbit

Antigen :

Choline Acetyltransferase (ChAT)

Conjugate :

Serum

Product Description :

Anti-Choline Acetyltransferase (ChAT) Antibody

Cross Reactivity :

Human;Rat;Mouse;Bat;Feline;Monkey

Background :

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

ALT Names :

Choline acetylase;acetyl CoA:choline O-acetyltransferase;choline acetyltransferase

Immunogen :

Human placental enzyme.

Specificity :

Cholinergic neurons in brain and central nervous systems.

Package Size :

50 µL

Uses :

Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.