LMX-1 Antibody | EMD Millipore AB10533 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

LMX-1 Antibody

catalog :

AB10533

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, ChIP-Seq

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...; fig 2c	Wizeman J, Guo Q, Wilion E, LI J. Specification of diverse cell types during early neurogenesis of the mouse cerebellum. elife. 2019;8: pubmed publisher
immunocytochemistry; human; loading ...; fig 1f	Playne R, Jones K, Connor B. Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors. J Stem Cells Regen Med. 2018;14:34-44 pubmed
immunohistochemistry; human; fig 7	La Manno G, Gyllborg D, Codeluppi S, Nishimura K, Salto C, Zeisel A, et al. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells. Cell. 2016;167:566-580.e19 pubmed publisher
immunocytochemistry; human; 1:250; fig 1	Borgs L, Peyre E, Alix P, Hanon K, Grobarczyk B, Godin J, et al. Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects. Sci Rep. 2016;6:33377 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1b	Doucet Beaupré H, Gilbert C, Profes M, Chabrat A, Pacelli C, Giguère N, et al. Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons. Proc Natl Acad Sci U S A. 2016;113:E4387-96 pubmed publisher
ChIP-Seq; zebrafish ; fig 5	Lin C, Erkek S, Tong Y, Yin L, Federation A, Zapatka M, et al. Active medulloblastoma enhancers reveal subgroup-specific cellular origins. Nature. 2016;530:57-62 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 5	Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, et al. Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 2016;409:55-71 pubmed publisher
	Adler A, Cardoso T, Nolbrant S, Mattsson B, Hoban D, Jarl U, et al. hESC-Derived Dopaminergic Transplants Integrate into Basal Ganglia Circuitry in a Preclinical Model of Parkinson's Disease. Cell Rep. 2019;28:3462-3473.e5 pubmed publisher
	Kishinevsky S, Wang T, Rodina A, Chung S, Xu C, Philip J, et al. HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain dopamine neurons. Nat Commun. 2018;9:4345 pubmed publisher
	Huang Y, Hill J, Yatteau A, Wong L, Jiang T, Petrovic J, et al. Reciprocal Negative Regulation Between Lmx1a and Lmo4 Is Required for Inner Ear Formation. J Neurosci. 2018;38:5429-5440 pubmed publisher
	Jovanovic V, Salti A, Tilleman H, Zega K, Jukic M, Zou H, et al. BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells. J Neurosci. 2018;38:1662-1676 pubmed publisher
	Haldipur P, Dang D, Aldinger K, Janson O, Guimiot F, Adle Biasette H, et al. Phenotypic outcomes in Mouse and Human Foxc1 dependent Dandy-Walker cerebellar malformation suggest shared mechanisms. elife. 2017;6: pubmed publisher
	Watanabe M, Fung E, Chan F, Wong J, Coutts M, Monuki E. BMP4 acts as a dorsal telencephalic morphogen in a mouse embryonic stem cell culture system. Biol Open. 2016;5:1834-1843 pubmed publisher
	Xia N, Zhang P, Fang F, Wang Z, Rothstein M, Angulo B, et al. Transcriptional comparison of human induced and primary midbrain dopaminergic neurons. Sci Rep. 2016;6:20270 pubmed publisher

product information

Catalog Number :

AB10533

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-LMX-1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q04650

Host Name :

Rabbit

Antigen :

LMX-1

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-LMX-1 Antibody

Cross Reactivity :

Mouse;Rat;Hamster

Background :

LMX-1 is a transcription factor that belongs to the LIM-homeodomain (LIM-HD) transcription factor family. It is induced within the ventral midbrain as a response to early signaling. LMX-1 is a necessary component for the development of the roof plate and for CNS dorsal cell date specification and the development of vertebrae. It is Expressed in roof plate, an organizational locus found in the dorsal CNS that functions to moderate the differentiation and specification of adjacent neurons through the secretion of signaling molecules such as WNT and BMP. Recent studies have indicated that LMX-1 has high potential for stimulating the production of mesencephalic dopamine neurons(mesDA) for the therapeutic treatment Parkinson disease, a condition characterized by dopaminergic neurotransmission decline resulting from the progression of mesDA neuronal degeneration within the substantia nigra pars compacta (SN).

ALT Names :

LIM homeobox transcription factor 1, alpha;LIM/homeobox protein 1.1;LIM/homeobox protein LMX1A

Immunogen :

GST-tagged recombinant protein corresponding to the C-terminus of hamster LMX-1.

Specificity :

This antibody recognizes LMX-1.

Package Size :

100 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.