PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) | EMD Millipore 676604-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6)

catalog :

676604-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5d	Li M, Quan C, Toth R, Campbell D, MacKintosh C, Wang H, et al. Fasting and Systemic Insulin Signaling Regulate Phosphorylation of Brain Proteins That Modulate Cell Morphology and Link to Neurological Disorders. J Biol Chem. 2015;290:30030-41 pubmed publisher

product information

Catalog Number :

676604-100UG

Category :

Antibodies

Subcategory :

Neuroscience

Product Name :

PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

7408 (Hu), 22323 (Ms), 361517 (Rt)

Host Name :

Clone :

5C6

Conjugate :

Purified

Isotype :

IgG1

Product Description :

PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6)

Cross Reactivity :

Human, Mouse

Background :

VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser157 in VASP can be used as a measure of PKA activity.

ALT Names :

AA107290, MGC127789, vasodilator-stimulated phosphoprotein

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 157 phosphorylation site of human VASP

Specificity :

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser 157 .

Package Size :

100 ug

Uses :

ELISA, WB, IC, IP

Storage :

Reconstitute in 1 ml sterile, dH 2 O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.