PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) | EMD Millipore 569372-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6)

catalog :

569372-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

569372-100UG

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

6714 (Hu), 20779 (Ms), 83805 (Rt)

Host Name :

Clone :

9A6

Conjugate :

Purified

Isotype :

IgG1

Product Description :

PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6)

Cross Reactivity :

Human, Mouse, Rat

Background :

Src belongs to a family of 9 tyrosine kinases which regulate cellular responses to extracellular stimuli. Src is a non-receptor protein tyrosine kinase that transduces signals that are involved in the control of a variety of cellular processes such as proliferation, differentiation, motility, and adhesion. Src is normally maintained in an inactive state, but can be activated transiently during cellular events such as mitosis, or constitutively by abnormal events such as mutation (i.e. v-Src and some human cancers). Src becomes activated upon dephosphorylation of Tyr527 which leads to a conformational change of the Src molecule. This allows binding to other proteins via the SH2 and SH3 domain. Activated Src phosphorylates a variety of substrates involved in the mitogenic signaling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication and RNA processing.

ALT Names :

ASV, AW259666, C-SRC, NSRC, p60 SRC, p60v-Src, PP60, PP60 SRC, Pp60/c-Src, PP60C-SRC, SRC1, TVHUSC, V-SRC, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr 418 phosphorylation site of human Src

Specificity :

Recognizes the ~60 kDa Src protein phosphorylated at Tyr 418 (human, rat), Tyr 423 (mouse), and Tyr 416 (chicken, v-Src).

Package Size :

100 ug

Uses :

ELISA, WB, IC

Storage :

Reconstitute in 1 ml sterile dH 2 O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.