PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10) | EMD Millipore 566807-1SET product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10)

catalog :

566807-1SET

quantity :

1 set

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA

citations: 1

Reference
Hanscom C, Talkowski M. Design of large-insert jumping libraries for structural variant detection using Illumina sequencing. Curr Protoc Hum Genet. 2014;80:7.22.1-9 pubmed publisher

product information

Catalog Number :

566807-1SET

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

6464 (Hu), 20416 (Ms), 85385 (Rt)

Host Name :

Clone :

6E10

Conjugate :

Purified

Isotype :

IgG1

Product Description :

PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10)

Cross Reactivity :

Human, Mouse

Background :

Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: Shc/p46, Shc/p52 and Shc/p66. Shc/p46 and Shc/p52 are found in every cell type, whereas Shc/p66 expression varies in different tissues. Shc proteins are phosphorylated by receptor tyrosine kinases and interact with phosphotyrosine residues on these receptors via the shc PTB and/or SH2 domains. The major tyrosine phosphorylation sites are tyrosines 239/240 as well as tyrosine 317. The Shc/p66 protein contains an additional N-terminal protein domain that is phosphorylated at serine 36. It is suggested that only the serine-phosphorylated Shc/p66 can interact with GRB2. Moreover, expression of Shc/p66 accelerates the inactivation of MAP kinases after EGF stimulation, whereas Shc/p46 and Shc/p52 enhance MAP kinase activity.

ALT Names :

FLJ26504, p52SHC, P66, p66SHC, SHC, Shc (46 kDa isoform), Shc p52, Shc p52 isoform, SHC P66, SHC P66 ISOFORM, SHCA, SHC (Src homology 2 domain containing) transforming protein 1

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 36 phosphorylation site of human Shc/p66

Specificity :

Recognizes the Shc/p66 protein phosphorylated on Ser 36 .

Package Size :

1 set

Uses :

ELISA, WB

Storage :

Reconstitute the antibody with 1 ml dH 2 O for at least 15 min at room temperature until completely dissolved. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the positive control with 125 ul H 2 O until completely dissolved. After complete solubilization, add 125 ul SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.