PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb | EMD Millipore 553009-10T product information

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product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb

catalog :

553009-10T

quantity :

10 test

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

Catalog Number :

553009-10T

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb

Product Type :

Antibodies

Clonality :

Polyclonal

Gene ID :

5894 (Hu), 110157 (Ms), 24703 (Rt)

Host Name :

Conjugate :

Purified

Isotype :

IgG

Product Description :

PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb

Cross Reactivity :

Human, Mouse, Rat

Background :

The raf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule. In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity. C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV. The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of ~74 kDa. Raf is widely expressed and plays an important role in cell proliferation and differentiation. Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus. Activation of raf itself is dependent on tyrosine (341) phosphorylation. Tyrosine phosphorylation is accomplished by Src tyrosine kinase.

ALT Names :

C-RAF, C-RAF1, D830050J10Rik, MGC102375, MURINE LEUKEMIA VIRAL ONCOGENE HOMOLOG1, NS5, Raf, RAF KINASE, v-Raf, v-raf-1 murine leukemia viral oncogene homolog 2

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr 340 and Tyr 341 , phosphorylation sites of human c-Raf (Raf-1)

Specificity :

Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr 340/341 .

Package Size :

10 test

Uses :

WB, IC

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.