EMD Millipore

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

chemical

NBD-Binding Peptide II, Cell Permeable, CTP-NBD Calbiochem 5 MG

480026-5MG

5 MG

Inflammation/Immunology

480026-5MG

NBD-Binding Peptide II, Cell Permeable, CTP-NBD Calbiochem 5 MG

Inhibitors

NBD-Binding Peptide II, Cell Permeable, CTP-NBD Calbiochem 5 MG

A cell-permeable cytoplasmic transduction peptide (CTP, YGRRARRRARR) fused to the NEMO binding domain peptide (NBD, TALDWSWLQTE) that acts as a potent inhibitor of TNF&alpha, -stimulated NF-&kappa, B activity (IC50 ~ 50 uM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents IkappaBalpha phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5 mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Antennapedia fused NBD cell-permeable peptide is also available (Cat. No. 480025).Peptide sequence: Ac-YGRRARRRARRTALDWSWLQTE-NH2

NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD

White solid. Packaged under inert gas.

≥97% by HPLC

5 MG

+2°C to +8°C. Hygroscopic. Protect from light. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up 6 months at -20°C.