PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) | EMD Millipore 324864-1SET product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2)

catalog :

324864-1SET

quantity :

1 set

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunoprecipitation

product information

Catalog Number :

324864-1SET

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

1956 (Hu), 13649 (Ms), 24329 (Rt)

Host Name :

Clone :

9H2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2)

Cross Reactivity :

Human, Mouse, Rat

Background :

Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues. The major site of tyrosine phosphorylation is tyrosine 1173.

ALT Names :

EGF RECEPTOR, EGF-TK, EGFR1, EGFRec, ER2, ERBB, ERBB1, Errp, HER1, MENA, PIG61, wa-2, Wa5, epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Immunogen :

a synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR

Specificity :

Recognizes the ~170 kDa EGFR protein phosphorylated on Tyr 1173 .

Package Size :

1 set

Uses :

ELISA, WB, IP

Storage :

Reconstitute the antibody with 1 ml H 2 O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 ul H 2 O. Add 200 ul SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).