This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
catalog :
17-672
quantity :
25 assays
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8WG16

The same clone is also sold as:
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 30
Published Application/Species/Sample/DilutionReference
  • chromatin immunoprecipitation; human; loading ...; fig 2a
Nguyen K, Das B, Dobrowolski C, Karn J. Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency. MBio. 2017;8: pubmed publisher
Gopalakrishnan R, Marr S, Kingston R, Winston F. A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation. Nucleic Acids Res. 2019;47:3888-3903 pubmed publisher
Castellaro A, Rodriguez Baili M, Di Tada C, Gil G. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel). 2019;11: pubmed publisher
Dunker W, Song Y, Zhao Y, Karijolich J. FUS Negatively Regulates Kaposi's Sarcoma-Associated Herpesvirus Gene Expression. Viruses. 2018;10: pubmed publisher
Rozenberg J, Taylor J, Mack C. RBPJ binds to consensus and methylated cis elements within phased nucleosomes and controls gene expression in human aortic smooth muscle cells in cooperation with SRF. Nucleic Acids Res. 2018;46:8232-8244 pubmed publisher
Watts B, Wittmann S, Wery M, Gautier C, Kus K, Birot A, et al. Histone deacetylation promotes transcriptional silencing at facultative heterochromatin. Nucleic Acids Res. 2018;46:5426-5440 pubmed publisher
Mo X, Zhang H, Preston S, Martin K, Zhou B, Vadalia N, et al. Interferon-γ Signaling in Melanocytes and Melanoma Cells Regulates Expression of CTLA-4. Cancer Res. 2018;78:436-450 pubmed publisher
Lai B, Lee J, Jang Y, Wang L, Peng W, Ge K. MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis. Nucleic Acids Res. 2017;45:6388-6403 pubmed publisher
Hang S, Gergen J. Different modes of enhancer-specific regulation by Runt and Even-skipped during Drosophila segmentation. Mol Biol Cell. 2017;28:681-691 pubmed publisher
Tran P, Kennedy B, Lien Y, Simmons R, Georgieff M. Fetal iron deficiency induces chromatin remodeling at the Bdnf locus in adult rat hippocampus. Am J Physiol Regul Integr Comp Physiol. 2015;308:R276-82 pubmed publisher
Yang S, Li W, Qi S, Gai K, Chen Y, Suo J, et al. The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region. Nucleic Acids Res. 2014;42:6183-95 pubmed publisher
Hérissant L, Moehle E, Bertaccini D, Van Dorsselaer A, Schaeffer Reiss C, Guthrie C, et al. H2B ubiquitylation modulates spliceosome assembly and function in budding yeast. Biol Cell. 2014;106:126-38 pubmed publisher
Lenstra T, Tudek A, Clauder S, Xu Z, Pachis S, van Leenen D, et al. The role of Ctk1 kinase in termination of small non-coding RNAs. PLoS ONE. 2013;8:e80495 pubmed publisher
Zhang Y, Vastenhouw N, Feng J, Fu K, Wang C, Ge Y, et al. Canonical nucleosome organization at promoters forms during genome activation. Genome Res. 2014;24:260-6 pubmed publisher
Mukundan B, Ansari A. Srb5/Med18-mediated termination of transcription is dependent on gene looping. J Biol Chem. 2013;288:11384-94 pubmed publisher
Lim C, Reversade B, Knowles B, Solter D. Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus. Development. 2013;140:853-60 pubmed publisher
Endoh M, Endo T, Endoh T, Isono K, Sharif J, Ohara O, et al. Histone H2A mono-ubiquitination is a crucial step to mediate PRC1-dependent repression of developmental genes to maintain ES cell identity. PLoS Genet. 2012;8:e1002774 pubmed publisher
Siuda D, Zechner U, El Hajj N, Prawitt D, Langer D, Xia N, et al. Transcriptional regulation of Nox4 by histone deacetylases in human endothelial cells. Basic Res Cardiol. 2012;107:283 pubmed publisher
Devaiah B, Lewis B, Cherman N, Hewitt M, Albrecht B, Robey P, et al. BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II carboxy-terminal domain. Proc Natl Acad Sci U S A. 2012;109:6927-32 pubmed publisher
St Amour C, Sansó M, Bösken C, Lee K, Larochelle S, Zhang C, et al. Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition. Mol Cell Biol. 2012;32:2372-83 pubmed publisher
Ni Z, Olsen J, Guo X, Zhong G, Ruan E, Marcon E, et al. Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B. Transcription. 2011;2:237-42 pubmed publisher
Bah A, Wischnewski H, Shchepachev V, Azzalin C. The telomeric transcriptome of Schizosaccharomyces pombe. Nucleic Acids Res. 2012;40:2995-3005 pubmed publisher
Hwang J, Saffert R, Kalejta R. Elongin B-mediated epigenetic alteration of viral chromatin correlates with efficient human cytomegalovirus gene expression and replication. MBio. 2011;2:e00023-11 pubmed publisher
Zediak V, Johnnidis J, Wherry E, Berger S. Cutting edge: persistently open chromatin at effector gene loci in resting memory CD8+ T cells independent of transcriptional status. J Immunol. 2011;186:2705-9 pubmed publisher
Garcia A, Rosonina E, Manley J, Calvo O. Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation. Mol Cell Biol. 2010;30:5180-93 pubmed publisher
Reynoird N, Schwartz B, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, et al. Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains. EMBO J. 2010;29:2943-52 pubmed publisher
Alder O, Lavial F, Helness A, Brookes E, Pinho S, Chandrashekran A, et al. Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development. 2010;137:2483-92 pubmed publisher
van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher
Malik S, Jiang S, Garee J, Verdin E, Lee A, O Malley B, et al. Histone deacetylase 7 and FoxA1 in estrogen-mediated repression of RPRM. Mol Cell Biol. 2010;30:399-412 pubmed publisher
Spencer C, Kruhlak M, Jenkins H, Sun X, Bazett Jones D. Mitotic transcription repression in vivo in the absence of nucleosomal chromatin condensation. J Cell Biol. 2000;150:13-26 pubmed
product information
Catalog Number :
17-672
Subcategory :
Epigenetics & Nuclear Function
Product Name :
ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P24928
Host Name :
Mouse
Antigen :
RNA Pol II
Clone :
8WG16
Conjugate :
Ascites
Isotype :
IgG2a
Product Description :
ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
Cross Reactivity :
Human;Mouse;Rat;Yeast ( S. cerevisiae )
ALT Names :
DNA directed RNA polymerase II polypeptide A ;DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit ;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA polymerase II subunit B1;polymerase (RNA) II (DNA directed) polypeptide A (220kD);polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Immunogen :
RNA Polymerase II purified from wheat germ extract.
Specificity :
Recognizes RNA polymerase II, Mr ~220 kDa.
Package Size :
25 assays
Uses :
Western Blotting;Immunohistochemistry;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.