ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

catalog :

17-662

quantity :

25 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC22

The same clone is also sold as:

Cell Signaling Technology: 3147
Invitrogen: 14-9867-80, 50-9867-82, 14-9867-37, 14-9867-82
Active Motif: 39875

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq, RNA immunoprecipitation, western blot knockout validation

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig s1b chromatin immunoprecipitation; human; loading ...; fig 2a	Nguyen K, Das B, Dobrowolski C, Karn J. Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency. MBio. 2017;8: pubmed publisher
RNA immunoprecipitation; human; fig 7b chromatin immunoprecipitation; human; loading ...; fig 8c	Qi F, Liu X, Wu H, Yu X, Wei C, Huang X, et al. Long noncoding AGAP2-AS1 is activated by SP1 and promotes cell proliferation and invasion in gastric cancer. J Hematol Oncol. 2017;10:48 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 5d	Jin Y, Huo B, Fu X, Hao T, Zhang Y, Guo Y, et al. LSD1 collaborates with EZH2 to regulate expression of interferon-stimulated genes. Biomed Pharmacother. 2017;88:728-737 pubmed publisher
immunoprecipitation; mouse; loading ...; fig 2d	Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, et al. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression. Proc Natl Acad Sci U S A. 2016;113:10370-5 pubmed publisher
immunoprecipitation; human; fig 5 immunocytochemistry; human; fig 3 western blot; human; fig 5	Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher
ChIP-Seq; mouse; fig st2 western blot; mouse; fig s10	Stelloh C, Reimer M, Pulakanti K, Blinka S, Peterson J, Pinello L, et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin. 2016;9:14 pubmed publisher
ChIP-Seq; mouse; fig 3	Fei Q, Yang X, Jiang H, Wang Q, Yu Y, Yu Y, et al. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells. Genome Res. 2015;25:1325-35 pubmed publisher
chromatin immunoprecipitation; human; fig 5 western blot; human; fig s2	Rao R, Dhele N, Cheemadan S, Ketkar A, Jayandharan G, Palakodeti D, et al. Ezh2 mediated H3K27me3 activity facilitates somatic transition during human pluripotent reprogramming. Sci Rep. 2015;5:8229 pubmed publisher
chromatin immunoprecipitation; human	Tange S, Oktyabri D, Terashima M, Ishimura A, Suzuki T. JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. PLoS ONE. 2014;9:e115684 pubmed publisher
western blot; mouse	Kao T, Liao H, Wolf D, Tai K, Chuang C, Lee H, et al. Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells. J Virol. 2014;88:10680-95 pubmed publisher
western blot; human	Svedlund J, Barazeghi E, Stalberg P, Hellman P, Akerstrom G, Björklund P, et al. The histone methyltransferase EZH2, an oncogene common to benign and malignant parathyroid tumors. Endocr Relat Cancer. 2014;21:231-9 pubmed publisher
chromatin immunoprecipitation; human	Basu A, Wilkinson F, Colavita K, Fennelly C, Atchison M. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids Res. 2014;42:2208-23 pubmed publisher
chromatin immunoprecipitation; mouse	Veazey K, Carnahan M, Muller D, Miranda R, Golding M. Alcohol-induced epigenetic alterations to developmentally crucial genes regulating neural stemness and differentiation. Alcohol Clin Exp Res. 2013;37:1111-22 pubmed publisher
	Oktyabri D, Tange S, Terashima M, Ishimura A, Suzuki T. EED regulates epithelial-mesenchymal transition of cancer cells induced by TGF-Î². Biochem Biophys Res Commun. 2014;453:124-30 pubmed publisher
	Reddington J, Perricone S, Nestor C, Reichmann J, Youngson N, Suzuki M, et al. Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes. Genome Biol. 2013;14:R25 pubmed publisher
	Saladi S, Wong P, Trivedi A, Marathe H, Keenen B, Aras S, et al. BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF to activate the melanoma inhibitor of apoptosis gene. Pigment Cell Melanoma Res. 2013;26:377-91 pubmed publisher
	Corvetta D, Chayka O, Gherardi S, D Acunto C, Cantilena S, Valli E, et al. Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: functional and therapeutic implications. J Biol Chem. 2013;288:8332-41 pubmed publisher
	Svedlund J, Koskinen Edblom S, Marquez V, Akerstrom G, Björklund P, Westin G. Hypermethylated in cancer 1 (HIC1), a tumor suppressor gene epigenetically deregulated in hyperparathyroid tumors by histone H3 lysine modification. J Clin Endocrinol Metab. 2012;97:E1307-15 pubmed publisher
	Ren G, Baritaki S, Marathe H, Feng J, Park S, Beach S, et al. Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer. Cancer Res. 2012;72:3091-104 pubmed publisher
	Zhao J, Yu J, Runkle C, Wu L, Hu M, Wu D, et al. Cooperation between Polycomb and androgen receptor during oncogenic transformation. Genome Res. 2012;22:322-31 pubmed publisher
	Zhang Y, Yang X, Gui B, Xie G, Zhang D, Shang Y, et al. Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27. J Biol Chem. 2011;286:42414-25 pubmed publisher
	Yang F, Zhang L, Huo X, Yuan J, Xu D, Yuan S, et al. Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans. Hepatology. 2011;54:1679-89 pubmed publisher
	Negishi M, Saraya A, Mochizuki S, Helin K, Koseki H, Iwama A. A novel zinc finger protein Zfp277 mediates transcriptional repression of the Ink4a/arf locus through polycomb repressive complex 1. PLoS ONE. 2010;5:e12373 pubmed publisher
	Yang X, Karuturi R, Sun F, Aau M, Yu K, Shao R, et al. CDKN1C (p57) is a direct target of EZH2 and suppressed by multiple epigenetic mechanisms in breast cancer cells. PLoS ONE. 2009;4:e5011 pubmed publisher

product information

Catalog Number :

17-662

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q15910

Host Name :

Mouse

Antigen :

EZH2

Isotype :

IgG2a

Product Description :

ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Mouse;Vertebrates;Rat

ALT Names :

Enhancer of zeste homolog 2;Lysine N-methyltransferase 6 ;enhancer of zeste 2;enhancer of zeste homolog 2 ;ENX-1

Immunogen :

EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.

Specificity :

Recognizes EZH2, MW: ~85-100 kDa.

Package Size :

25 assays

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.