ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

catalog :

17-656

quantity :

25 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F1.2

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 5

Reference
Watanabe M, Terasawa M, Miyano K, Yanagihara T, Uruno T, Sanematsu F, et al. DOCK2 and DOCK5 act additively in neutrophils to regulate chemotaxis, superoxide production, and extracellular trap formation. J Immunol. 2014;193:5660-7 pubmed publisher
Siegle J, Basin A, Sastre Perona A, Yonekubo Y, Brown J, Sennett R, et al. SOX2 is a cancer-specific regulator of tumour initiating potential in cutaneous squamous cell carcinoma. Nat Commun. 2014;5:4511 pubmed publisher
Scardigli R, Capelli P, Vignone D, Brandi R, Ceci M, La Regina F, et al. Neutralization of nerve growth factor impairs proliferation and differentiation of adult neural progenitors in the subventricular zone. Stem Cells. 2014;32:2516-28 pubmed publisher
Chou Y, Lee C, Hsiao S, Lin S, Lin S, Chung C, et al. The emerging role of SOX2 in cell proliferation and survival and its crosstalk with oncogenic signaling in lung cancer. Stem Cells. 2013;31:2607-19 pubmed publisher
Tan G, Krammer F, Eggink D, Kongchanagul A, Moran T, Palese P. A pan-H1 anti-hemagglutinin monoclonal antibody with potent broad-spectrum efficacy in vivo. J Virol. 2012;86:6179-88 pubmed publisher

product information

Catalog Number :

17-656

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P48431

Host Name :

Mouse

Antigen :

Sox-2

Clone :

6F1.2

Conjugate :

Purified

Isotype :

IgG2bκ

Product Description :

ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Mouse

Background :

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.

ALT Names :

Transcription factor SOX-2;SOX2

Immunogen :

Recombinant GST Fusion protein.

Specificity :

Recognizes Sox-2, MW: ~34 kDa.

Package Size :

25 assays

Uses :

Western Blotting;Dot Blot;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.