ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

catalog :

17-650

quantity :

25 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Woo Park J, Kim K, Kim J, Chae Y, Jeong O, Seo S. RE-IIBP Methylates H3K79 and Induces MEIS1-mediated Apoptosis via H2BK120 Ubiquitination by RNF20. Sci Rep. 2015;5:12485 pubmed publisher
	Li X, Seidel C, Szerszen L, Lange J, Workman J, Abmayr S. Enzymatic modules of the SAGA chromatin-modifying complex play distinct roles in Drosophila gene expression and development. Genes Dev. 2017;31:1588-1600 pubmed publisher
	Lee B, Lee J, Shen W, Rhee C, Chung H, Kim J. Fbxl19 recruitment to CpG islands is required for Rnf20-mediated H2B mono-ubiquitination. Nucleic Acids Res. 2017;45:7151-7166 pubmed publisher
	Jung I, Kim S, Kim M, Han Y, Kim Y, Kim D, et al. H2B monoubiquitylation is a 5'-enriched active transcription mark and correlates with exon-intron structure in human cells. Genome Res. 2012;22:1026-35 pubmed publisher
	Hwang J, Saffert R, Kalejta R. Elongin B-mediated epigenetic alteration of viral chromatin correlates with efficient human cytomegalovirus gene expression and replication. MBio. 2011;2:e00023-11 pubmed publisher

product information

Catalog Number :

17-650

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P62807

Host Name :

Mouse

Antigen :

Ubiquityl-Histone H2B

Conjugate :

Protein G Purified

Isotype :

IgG2aκ

Product Description :

ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Mouse;Human;Vertebrates;Rat;Green Plants

ALT Names :

H2BUb;Histone H2B (ubiquityl);H2B histone family, member L;H2B.1 A;histone 1, H2bc;histone cluster 1, H2bc

Immunogen :

Immunogen used was a branched peptide corresponding to the ubiquitination site on human histone H2B.

Specificity :

Recognizes histone H2B ubiquitinated on lysine 120 but not free ubiquitin or histone H2B in its unmodified state.

Package Size :

25 assays

Uses :

Western Blotting;Immunofluorescence;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.