This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
catalog :
17-614
quantity :
25 assays
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, fruit fly
application :
western blot, chromatin immunoprecipitation, ChIP-Seq
citations: 66
Published Application/Species/Sample/DilutionReference
  • ChIP-Seq; human; loading ...; fig 2b
Yukawa M, Jagannathan S, Vallabh S, Kartashov A, Chen X, Weirauch M, et al. AP-1 activity induced by co-stimulation is required for chromatin opening during T cell activation. J Exp Med. 2020;217: pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 2f
Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 7a
Patke A, Murphy P, Onat O, Krieger A, Ozcelik T, Campbell S, et al. Mutation of the Human Circadian Clock Gene CRY1 in Familial Delayed Sleep Phase Disorder. Cell. 2017;169:203-215.e13 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4b
Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Hypermethylated LTR retrotransposon exhibits enhancer activity. Epigenetics. 2017;12:226-237 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig s5j
Choi Y, Lin C, Risso D, Chen S, Kim T, Tan M, et al. Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells. Science. 2017;355: pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 3b
Zeng X, Willi M, Shin H, Hennighausen L, Wang C. Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5. Cell Rep. 2016;17:3333-3346 pubmed publisher
  • chromatin immunoprecipitation; human; fig 3b
Kalchschmidt J, Bashford Rogers R, Paschos K, Gillman A, Styles C, Kellam P, et al. Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells. J Exp Med. 2016;213:921-8 pubmed publisher
  • ChIP-Seq; fruit fly ; fig 3
Kwon S, Grisan V, Jang B, Herbert J, Badenhorst P. Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators. PLoS Genet. 2016;12:e1005969 pubmed publisher
  • western blot; human; tbl 1
Richarson A, Scott D, Zagnitko O, Aza Blanc P, Chang C, Russler Germain D. Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation. elife. 2016;5:e10860 pubmed publisher
  • ChIP-Seq; mouse; fig 3
Metser G, Shin H, Wang C, Yoo K, Oh S, Villarino A, et al. An autoregulatory enhancer controls mammary-specific STAT5 functions. Nucleic Acids Res. 2016;44:1052-63 pubmed publisher
  • chromatin immunoprecipitation; human; fig 5b
Wang Y, Zhong H, Xie X, Chen C, Huang D, Shen L, et al. Long noncoding RNA derived from CD244 signaling epigenetically controls CD8+ T-cell immune responses in tuberculosis infection. Proc Natl Acad Sci U S A. 2015;112:E3883-92 pubmed publisher
  • chromatin immunoprecipitation; rat; fig 5J
  • western blot; rat; 1:1000; fig 5I
Rui Y, Xu L, Chen R, Zhang T, Lin S, Hou Y, et al. Epigenetic memory gained by priming with osteogenic induction medium improves osteogenesis and other properties of mesenchymal stem cells. Sci Rep. 2015;5:11056 pubmed publisher
  • chromatin immunoprecipitation; human
Ren G, Cui K, Zhang Z, Zhao K. Division of labor between IRF1 and IRF2 in regulating different stages of transcriptional activation in cellular antiviral activities. Cell Biosci. 2015;5:17 pubmed publisher
  • chromatin immunoprecipitation; rat; fig 3
Koo J, Mazei Robison M, LaPlant Q, Egervári G, Braunscheidel K, Adank D, et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci. 2015;18:415-22 pubmed publisher
  • chromatin immunoprecipitation; human; fig 5.b,f
Zimmermann M, Aguilera F, Castellucci M, Rossato M, Costa S, Lunardi C, et al. Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils. Nat Commun. 2015;6:6061 pubmed publisher
  • ChIP-Seq; mouse; fig s2
Bae W, Kang K, Yu J, Yoo K, Factor V, Kaji K, et al. The methyltransferases enhancer of zeste homolog (EZH) 1 and EZH2 control hepatocyte homeostasis and regeneration. FASEB J. 2015;29:1653-62 pubmed publisher
  • chromatin immunoprecipitation; human
Rochman M, Kartashov A, Caldwell J, Collins M, Stucke E, Kc K, et al. Neurotrophic tyrosine kinase receptor 1 is a direct transcriptional and epigenetic target of IL-13 involved in allergic inflammation. Mucosal Immunol. 2015;8:785-98 pubmed publisher
  • chromatin immunoprecipitation; human
Balmer N, Klima S, Rempel E, Ivanova V, Kolde R, Weng M, et al. From transient transcriptome responses to disturbed neurodevelopment: role of histone acetylation and methylation as epigenetic switch between reversible and irreversible drug effects. Arch Toxicol. 2014;88:1451-68 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
Ravnskjaer K, Hogan M, Lackey D, Tora L, Dent S, Olefsky J, et al. Glucagon regulates gluconeogenesis through KAT2B- and WDR5-mediated epigenetic effects. J Clin Invest. 2013;123:4318-28 pubmed publisher
  • chromatin immunoprecipitation; human
Murata T, Narita Y, Sugimoto A, Kawashima D, Kanda T, Tsurumi T. Contribution of myocyte enhancer factor 2 family transcription factors to BZLF1 expression in Epstein-Barr virus reactivation from latency. J Virol. 2013;87:10148-62 pubmed publisher
  • chromatin immunoprecipitation; human; 3 ul/rxn
Thijssen P, Tobi E, Balog J, Schouten S, Kremer D, El Bouazzaoui F, et al. Chromatin remodeling of human subtelomeres and TERRA promoters upon cellular senescence: commonalities and differences between chromosomes. Epigenetics. 2013;8:512-21 pubmed publisher
  • chromatin immunoprecipitation; mouse
Pei H, Wu X, Liu T, Yu K, Jelinek D, Lou Z. The histone methyltransferase MMSET regulates class switch recombination. J Immunol. 2013;190:756-63 pubmed publisher
  • chromatin immunoprecipitation; human
Weng M, Zimmer B, Pöltl D, Broeg M, Ivanova V, Gaspar J, et al. Extensive transcriptional regulation of chromatin modifiers during human neurodevelopment. PLoS ONE. 2012;7:e36708 pubmed publisher
  • chromatin immunoprecipitation; human
Murata T, Kondo Y, Sugimoto A, Kawashima D, Saito S, Isomura H, et al. Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells. J Virol. 2012;86:4752-61 pubmed publisher
Carter B, Ku W, Kang J, Hu G, Perrie J, Tang Q, et al. Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq). Nat Commun. 2019;10:3747 pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
G rard D, Schmidt F, Ginolhac A, Schmitz M, Halder R, Ebert P, et al. Temporal enhancer profiling of parallel lineages identifies AHR and GLIS1 as regulators of mesenchymal multipotency. Nucleic Acids Res. 2019;47:1141-1163 pubmed publisher
Malhotra N, Qi Y, Spidale N, Frascoli M, Miu B, Cho O, et al. SOX4 controls invariant NKT cell differentiation by tuning TCR signaling. J Exp Med. 2018;215:2887-2900 pubmed publisher
Gillman A, Parker G, Allday M, Bazot Q. Epstein-Barr Virus Nuclear Antigen 3C Inhibits Expression of COBLL1 and the ADAM28-ADAMDEC1 Locus via Interaction with the Histone Lysine Demethylase KDM2B. J Virol. 2018;92: pubmed publisher
Kurup J, Kidder B. Identification of H4K20me3- and H3K4me3-associated RNAs using CARIP-Seq expands the transcriptional and epigenetic networks of embryonic stem cells. J Biol Chem. 2018;293:15120-15135 pubmed publisher
Xu J, Kidder B. H4K20me3 co-localizes with activating histone modifications at transcriptionally dynamic regions in embryonic stem cells. BMC Genomics. 2018;19:514 pubmed publisher
Wang C, Jégu T, Chu H, Oh H, Lee J. SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X. Cell. 2018;174:406-421.e25 pubmed publisher
Xu J, Kidder B. KDM5B decommissions the H3K4 methylation landscape of self-renewal genes during trophoblast stem cell differentiation. Biol Open. 2018;7: pubmed publisher
Zheng Y, Xue M, Shen H, Li X, Ma D, Gong Y, et al. PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Cancer Res. 2018;78:3190-3206 pubmed publisher
Dobenecker M, Park J, Marcello J, McCabe M, Gregory R, Knight S, et al. Signaling function of PRC2 is essential for TCR-driven T cell responses. J Exp Med. 2018;215:1101-1113 pubmed publisher
Sarrach S, Huang Y, Niedermeyer S, Hachmeister M, Fischer L, Gille S, et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci Rep. 2018;8:1801 pubmed publisher
Bazot Q, Paschos K, Allday M. Epstein-Barr Virus (EBV) Latent Protein EBNA3A Directly Targets and Silences the STK39 Gene in B Cells Infected by EBV. J Virol. 2018;92: pubmed publisher
Wang H, Bender A, Wang P, Karaköse E, Inabnet W, Libutti S, et al. Insights into beta cell regeneration for diabetes via integration of molecular landscapes in human insulinomas. Nat Commun. 2017;8:767 pubmed publisher
Shintaku J, Peterson J, Talbert E, Gu J, Ladner K, Williams D, et al. MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-?B. Cell Rep. 2016;17:514-526 pubmed publisher
Luo K, Luo D, Wen H. Homeobox genes gain trimethylation of histone H3 lysine 4 in glioblastoma tissue. Biosci Rep. 2016;36: pubmed publisher
Vento Tormo R, Rodríguez Ubreva J, Lisio L, Islam A, Urquiza J, Hernando H, et al. NF-κB directly mediates epigenetic deregulation of common microRNAs in Epstein-Barr virus-mediated transformation of B-cells and in lymphomas. Nucleic Acids Res. 2014;42:11025-39 pubmed publisher
Heller J, Schjerven H, Li S, Lee A, Qiu J, Chen Z, et al. Restriction of IL-22-producing T cell responses and differential regulation of regulatory T cell compartments by zinc finger transcription factor Ikaros. J Immunol. 2014;193:3934-46 pubmed publisher
Hassan H, Carlson S, Abdallah I, Buttolph T, Glass K, Fandy T. Curcumin and dimethoxycurcumin induced epigenetic changes in leukemia cells. Pharm Res. 2015;32:863-75 pubmed publisher
Zhang L, Tang L, Wei J, Lao L, Gu W, Hu Q, et al. Extrauterine growth restriction on pulmonary vascular endothelial dysfunction in adult male rats: the role of epigenetic mechanisms. J Hypertens. 2014;32:2188-98; discussion 2198 pubmed publisher
Gao F, Ji G, Gao Z, Han X, Ye M, Yuan Z, et al. Direct ChIP-bisulfite sequencing reveals a role of H3K27me3 mediating aberrant hypermethylation of promoter CpG islands in cancer cells. Genomics. 2014;103:204-10 pubmed publisher
Pedersen M, Agger K, Laugesen A, Johansen J, Cloos P, Christensen J, et al. The demethylase JMJD2C localizes to H3K4me3-positive transcription start sites and is dispensable for embryonic development. Mol Cell Biol. 2014;34:1031-45 pubmed publisher
Meng L, Person R, Huang W, Zhu P, Costa Mattioli M, Beaudet A. Truncation of Ube3a-ATS unsilences paternal Ube3a and ameliorates behavioral defects in the Angelman syndrome mouse model. PLoS Genet. 2013;9:e1004039 pubmed publisher
Kang K, Yamaji D, Yoo K, Robinson G, Hennighausen L. Mammary-specific gene activation is defined by progressive recruitment of STAT5 during pregnancy and the establishment of H3K4me3 marks. Mol Cell Biol. 2014;34:464-73 pubmed publisher
Galhardo M, Sinkkonen L, Berninger P, Lin J, Sauter T, Heinaniemi M. Integrated analysis of transcript-level regulation of metabolism reveals disease-relevant nodes of the human metabolic network. Nucleic Acids Res. 2014;42:1474-96 pubmed publisher
Yokobayashi S, Liang C, Kohler H, Nestorov P, Liu Z, Vidal M, et al. PRC1 coordinates timing of sexual differentiation of female primordial germ cells. Nature. 2013;495:236-40 pubmed publisher
Yamaji D, Kang K, Robinson G, Hennighausen L. Sequential activation of genetic programs in mouse mammary epithelium during pregnancy depends on STAT5A/B concentration. Nucleic Acids Res. 2013;41:1622-36 pubmed publisher
Rebollo R, Miceli Royer K, Zhang Y, Farivar S, Gagnier L, Mager D. Epigenetic interplay between mouse endogenous retroviruses and host genes. Genome Biol. 2012;13:R89 pubmed publisher
Estecio M, Gallegos J, Dekmezian M, Lu Y, Liang S, Issa J. SINE retrotransposons cause epigenetic reprogramming of adjacent gene promoters. Mol Cancer Res. 2012;10:1332-42 pubmed publisher
Neault M, Mallette F, Vogel G, Michaud Levesque J, Richard S. Ablation of PRMT6 reveals a role as a negative transcriptional regulator of the p53 tumor suppressor. Nucleic Acids Res. 2012;40:9513-21 pubmed publisher
Fang T, Schaefer U, Mecklenbrauker I, Stienen A, Dewell S, Chen M, et al. Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response. J Exp Med. 2012;209:661-9 pubmed publisher
Agorogiannis E, Regateiro F, Howie D, Waldmann H, Cobbold S. Th17 cells induce a distinct graft rejection response that does not require IL-17A. Am J Transplant. 2012;12:835-45 pubmed publisher
Raynal N, Si J, Taby R, Gharibyan V, Ahmed S, Jelinek J, et al. DNA methylation does not stably lock gene expression but instead serves as a molecular mark for gene silencing memory. Cancer Res. 2012;72:1170-81 pubmed publisher
Rodriguez Ubreva J, Ciudad L, Gomez Cabrero D, Parra M, Bussmann L, di Tullio A, et al. Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes. Nucleic Acids Res. 2012;40:1954-68 pubmed publisher
Cohen C, Crome S, Macdonald K, Dai E, Mager D, Levings M. Human Th1 and Th17 cells exhibit epigenetic stability at signature cytokine and transcription factor loci. J Immunol. 2011;187:5615-26 pubmed publisher
Noordermeer D, Leleu M, Splinter E, Rougemont J, de Laat W, Duboule D. The dynamic architecture of Hox gene clusters. Science. 2011;334:222-5 pubmed publisher
Ren L, Shi M, Wang Y, Yang Z, Wang X, Zhao Z. CTCF and cohesin cooperatively mediate the cell-type specific interchromatin interaction between Bcl11b and Arhgap6 loci. Mol Cell Biochem. 2012;360:243-51 pubmed publisher
Li W, Han Y, Tao F, Chong K. Knockdown of SAMS genes encoding S-adenosyl-l-methionine synthetases causes methylation alterations of DNAs and histones and leads to late flowering in rice. J Plant Physiol. 2011;168:1837-43 pubmed publisher
Javierre B, Rodriguez Ubreva J, Al Shahrour F, Corominas M, Grana O, Ciudad L, et al. Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells. Mol Cancer Res. 2011;9:1139-51 pubmed publisher
Niu X, Zhang T, Liao L, Zhou L, Lindner D, Zhou M, et al. The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C. Oncogene. 2012;31:776-86 pubmed publisher
Maruo S, Zhao B, Johannsen E, Kieff E, Zou J, Takada K. Epstein-Barr virus nuclear antigens 3C and 3A maintain lymphoblastoid cell growth by repressing p16INK4A and p14ARF expression. Proc Natl Acad Sci U S A. 2011;108:1919-24 pubmed publisher
Wu T, Zhang X, Huang X, Yang Y, Hua X. Regulation of cyclin B2 expression and cell cycle G2/m transition by menin. J Biol Chem. 2010;285:18291-300 pubmed publisher
product information
Catalog Number :
17-614
Subcategory :
Epigenetics & Nuclear Function
Product Name :
ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q66I33
Host Name :
Rabbit
Antigen :
Trimethyl-Histone H3 (Lys4)
Conjugate :
Purified
Isotype :
IgG
Product Description :
ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
Cross Reactivity :
Human;Mouse;Mammals
ALT Names :
H3K4me3;Histone H3 (tri methyl K4)
Immunogen :
The trimethyl-histone H3 (Lys4) antibody is made against a BSA-conjugated, synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3
Specificity :
Trimethyl-Histone H3 (Lys4)
Package Size :
25 assays
Uses :
Western Blotting;Chromatin Immunoprecipitation (ChIP);ChIP-seq
Storage :
Stable for 1 year at -20°C from date of receipt
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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