CREB | EMD Millipore 17-600 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CREB

catalog :

17-600

quantity :

25 assays

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

NL904

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, EMSA, chromatin immunoprecipitation, ChIP-Seq

citations: 9

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; loading ...; fig 5d	Jana M, Ghosh S, Pahan K. Upregulation of Myelin Gene Expression by a Physically-Modified Saline via Phosphatidylinositol 3-Kinase-Mediated Activation of CREB: Implications for Multiple Sclerosis. Neurochem Res. 2018;43:407-419 pubmed publisher
western blot; mouse; fig s4i	Wang J, Telese F, Tan Y, Li W, Jin C, He X, et al. LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control. Nat Neurosci. 2015;18:1256-64 pubmed publisher
chromatin immunoprecipitation; human; fig 7c EMSA; human; fig 7a	Shi C, Zhang M, Tong M, Yang L, Pang L, Chen L, et al. miR-148a is Associated with Obesity and Modulates Adipocyte Differentiation of Mesenchymal Stem Cells through Wnt Signaling. Sci Rep. 2015;5:9930 pubmed publisher
ChIP-Seq; mouse; fig s2b	Telese F, Ma Q, Perez P, Notani D, Oh S, Li W, et al. LRP8-Reelin-Regulated Neuronal Enhancer Signature Underlying Learning and Memory Formation. Neuron. 2015;86:696-710 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 4 western blot; rat; 1:1000; fig s4	Koo J, Mazei Robison M, LaPlant Q, Egervári G, Braunscheidel K, Adank D, et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci. 2015;18:415-22 pubmed publisher
chromatin immunoprecipitation; human immunocytochemistry; human	Arampatzi P, Gialitakis M, Makatounakis T, Papamatheakis J. Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Nucleic Acids Res. 2013;41:2202-15 pubmed publisher
	Ma T, Barco A, Ratan R, Willis D. cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth. J Biol Chem. 2014;289:32914-25 pubmed publisher
	Matsui M, Chu Y, Zhang H, Gagnon K, Shaikh S, Kuchimanchi S, et al. Promoter RNA links transcriptional regulation of inflammatory pathway genes. Nucleic Acids Res. 2013;41:10086-109 pubmed publisher
	Huang H, Amin V, Gurin M, Wan E, Thorp E, Homma S, et al. Diet-induced obesity causes long QT and reduces transcription of voltage-gated potassium channels. J Mol Cell Cardiol. 2013;59:151-8 pubmed publisher

product information

Catalog Number :

17-600

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P16220

Host Name :

Rabbit

Antigen :

CREB

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene. The CREB and negative control cultured supernatant are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CREB associated chromatin.

ALT Names :

active transcription factor CREB;cAMP responsive element binding protein 1;cAMP-response element-binding protein-1;cAMP-responsive element-binding protein 1;transactivator protein

Immunogen :

CREB purified antibody is made against a KLH-conjugated synthetic peptide corre-sponding to amino acids 5-24 of human CREB.

Specificity :

Recognizes CREB, MW ~43 kDa.

Package Size :

25 assays

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.