ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set | EMD Millipore 17-10531 product information

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product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set

catalog :

17-10531

quantity :

25 assays

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 8c	Qi F, Liu X, Wu H, Yu X, Wei C, Huang X, et al. Long noncoding AGAP2-AS1 is activated by SP1 and promotes cell proliferation and invasion in gastric cancer. J Hematol Oncol. 2017;10:48 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 5d	Jin Y, Huo B, Fu X, Hao T, Zhang Y, Guo Y, et al. LSD1 collaborates with EZH2 to regulate expression of interferon-stimulated genes. Biomed Pharmacother. 2017;88:728-737 pubmed publisher
western blot; mouse; loading ...; fig 5a	Patrick N, Griggs C, Icenogle A, Gilpatrick M, Kadiyala V, Jaime Frias R, et al. Class I lysine deacetylases promote glucocorticoid-induced transcriptional repression through functional interaction with LSD1. J Steroid Biochem Mol Biol. 2017;167:1-13 pubmed publisher

product information

Catalog Number :

17-10531

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O60341

Host Name :

Rabbit

Antigen :

KDM1A

Conjugate :

Affinity Purified

Purification Method :

Affinity purified

Product Description :

ChIPAb+ LSD1 (KDM1A) Antibody - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human, Mouse, Rat

Background :

LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ LSD1 (KDM1A) set includes a LSD1 antibody, a Normal rabbit IgG and positive control primers which amplify a 85bp human prostate-specific antigen enhancer region. The LSD1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of LSD1 associated chromatin.

ALT Names :

Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2

Immunogen :

Histidine-tagged recombinant protein corresponding to full length LSD1.

Package Size :

25 assays

Uses :

Chromatin Immunoprecipitation (ChIP), Western Blotting, Immunoprecipitation

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.