ChIPAb+™ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set | EMD Millipore 17-10242 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+™ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set

catalog :

17-10242

quantity :

25 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

product information

Catalog Number :

17-10242

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+™ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Trimethyl-Histone H3 (Lys9)

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

ChIPAb+™ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

ALT Names :

H3K9me3;Histone H3 (tri methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated synthetic peptide corresponding to human Histone H3 trimethylated on Lys9.

Specificity :

This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is 27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.

Package Size :

25 assays

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.