ChIPAb+™ Phospho-Histone H3 (Ser28) | EMD Millipore 17-10140 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+™ Phospho-Histone H3 (Ser28)

catalog :

17-10140

quantity :

25 assays

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; fig st1	Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher

product information

Catalog Number :

17-10140

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+™ Phospho-Histone H3 (Ser28)

Product Type :

Antibodies

Clonality :

Polyclonal

Gene ID :

NP_003493

Host Name :

Conjugate :

Affinity Purified

Product Description :

ChIPAb+™ Phospho-Histone H3 (Ser28)

Cross Reactivity :

Human

Species Summary :

Human. Broad species cross-reactivity is expected.

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3S28P, Histone H3 (phospho S28), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

Immunogen :

BSA-conjugated, synthetic peptide corresponding to amino acids 25-37 of human Histone H3 (ARK[pS]APATGGVKK-C), with a C-terminal cysteine added to facilitate conjugation.

Specificity :

Recognizes Histone H3 phosphorylated at Ser28, Mr 17 kDa. BSA is observed, Mr 66 kDa, in longer exposures. Does not cross-react with Histone H3 phosphorylated at Serine 10.

Package Size :

25 assays

Uses :

ChIP, WB

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.