ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set | EMD Millipore 17-10131 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

catalog :

17-10131

quantity :

25 assays

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...	Salvi V, Vermi W, Gianello V, Lonardi S, Gagliostro V, Naldini A, et al. Dendritic cell-derived VEGF-A plays a role in inflammatory angiogenesis of human secondary lymphoid organs and is driven by the coordinated activation of multiple transcription factors. Oncotarget. 2016;7:39256-39269 pubmed publisher

product information

Catalog Number :

17-10131

Subcategory :

Cell Signaling

Product Name :

ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P16220

Host Name :

Rabbit

Antigen :

Phospho-CREB (Ser133)

Conjugate :

Purified

Isotype :

IgG

Product Description :

ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Rat;Mouse;Hamster

Background :

CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

ALT Names :

active transcription factor CREB;cAMP responsive element binding protein 1;cAMP-response element-binding protein-1;transactivator protein

Immunogen :

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.

Specificity :

Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.

Package Size :

25 assays

Uses :

Electrophoretic Mobility Shift Assay;Immunohistochemistry;Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.