ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set | EMD Millipore 17-10060 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set

catalog :

17-10060

quantity :

25 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, EMSA, chromatin immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 5a	Wang Y, Yun C, Gao B, Xu Y, Zhang Y, Wang Y, et al. The Lysine Acetyltransferase GCN5 Is Required for iNKT Cell Development through EGR2 Acetylation. Cell Rep. 2017;20:600-612 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 3a immunocytochemistry; human; 1:75; loading ...; fig 4a	Park S, Yi H, Suh N, Park Y, Koh J, Jeong S, et al. Inhibition of EHMT2/G9a epigenetically increases the transcription of Beclin-1 via an increase in ROS and activation of NF-?B. Oncotarget. 2016;7:39796-39808 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Caporali A, Meloni M, Nailor A, Mitić T, Shantikumar S, Riu F, et al. p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia. Nat Commun. 2015;6:8024 pubmed publisher
chromatin immunoprecipitation; mouse	Kumar V, Palermo R, Talora C, Campese A, Checquolo S, Bellavia D, et al. Notch and NF-kB signaling pathways regulate miR-223/FBXW7 axis in T-cell acute lymphoblastic leukemia. Leukemia. 2014;28:2324-35 pubmed publisher
chromatin immunoprecipitation; human	Wang Z, Wang B, Shi Y, Xu C, Xiao H, Ma L, et al. Oncogenic miR-20a and miR-106a enhance the invasiveness of human glioma stem cells by directly targeting TIMP-2. Oncogene. 2015;34:1407-19 pubmed publisher
chromatin immunoprecipitation; human	Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-?B and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher
	Johnston A, Simões Pires C, Thompson T, Suzuki M, Greally J. Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding. Nat Commun. 2019;10:3472 pubmed publisher
	Parikh N, Shuck R, Nguyen T, Herron A, Donehower L. Mouse tissues that undergo neoplastic progression after K-Ras activation are distinguished by nuclear translocation of phospho-Erk1/2 and robust tumor suppressor responses. Mol Cancer Res. 2012;10:845-55 pubmed publisher
	Byrd A, Aragon I, Brewer J. MicroRNA-30c-2* limits expression of proadaptive factor XBP1 in the unfolded protein response. J Cell Biol. 2012;196:689-98 pubmed publisher

product information

Catalog Number :

17-10060

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q04206

Host Name :

Mouse

Antigen :

NFκB p65 (RelA)

Conjugate :

Purified

Isotype :

IgG3

Product Description :

ChIPAb+ NFκB p65 (RelA) - ChIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Mouse;Rat

Background :

The transcription factor NFkappaB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFkappaB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFkappaB1 (p50 and its precursor p105), and NFkappaB2 (p52 and its precursor p100). NFkappaB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFkappaB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFkappaB is the most abundant in cells.

ALT Names :

Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Immunogen :

Peptide corresponding to human p65 coupled to BSA.

Specificity :

This antibody recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.

Package Size :

25 assays

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.