ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal | EMD Millipore 17-10032 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

catalog :

17-10032

quantity :

25 assays

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig s7	Valle García D, Qadeer Z, McHugh D, Ghiraldini F, Chowdhury A, Hasson D, et al. ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment. Epigenetics. 2016;11:398-414 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig s4d	Han P, Li W, Lin C, Yang J, Shang C, Nuernberg S, et al. A long noncoding RNA protects the heart from pathological hypertrophy. Nature. 2014;514:102-106 pubmed publisher
	Jangid R, Kelkar A, Muley V, Galande S. Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions. BMC Genomics. 2018;19:313 pubmed publisher
	Hassan H, Carlson S, Abdallah I, Buttolph T, Glass K, Fandy T. Curcumin and dimethoxycurcumin induced epigenetic changes in leukemia cells. Pharm Res. 2015;32:863-75 pubmed publisher

product information

Catalog Number :

17-10032

Subcategory :

Epigenetics & Nuclear Function

Product Name :

ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

Trimethyl-Histone H3 (Lys36)

Conjugate :

Purified

Isotype :

IgG

Product Description :

ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

Cross Reactivity :

Human;Chicken

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.

ALT Names :

H3K36me3;Histone H3 (tri methyl K36);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence . GVme3KKP…, in which me3K corresponds to human trimethyl-histone H3 (Lys36).

Specificity :

Recognizes Trimethyl-Histone H3 (Lys36), Mr ~17 kDa.

Package Size :

25 assays

Uses :

Chromatin Immunoprecipitation (ChIP);Western Blotting;Cell Function Assay;Dot Blot

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.