Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate | EMD Millipore 16-316 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

catalog :

16-316

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

4G10® and PY20

application :

western blot, ELISA

citations: 3

Reference
Yin C, Zhu B, Zhang T, Liu T, Chen S, Liu Y, et al. Pharmacological Targeting of STK19 Inhibits Oncogenic NRAS-Driven Melanomagenesis. Cell. 2019;176:1113-1127.e16 pubmed publisher
Tian W, Qu L, Meng L, Liu C, Wu J, Shou C. Phosphatase of regenerating liver-3 directly interacts with integrin ?1 and regulates its phosphorylation at tyrosine 783. BMC Biochem. 2012;13:22 pubmed publisher
Adwan T, Ohm A, Jones D, Humphries M, Reyland M. Regulated binding of importin-? to protein kinase C? in response to apoptotic signals facilitates nuclear import. J Biol Chem. 2011;286:35716-24 pubmed publisher

product information

Catalog Number :

16-316

Subcategory :

Cell Signaling

Product Name :

Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Varies

Host Name :

Mouse

Antigen :

Phosphotyrosine

Clone :

4G10® and PY20

Conjugate :

HRP

Isotype :

IgG2b

Product Description :

Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

Cross Reactivity :

All

Background :

The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone.

ALT Names :

phosphotyrosine

Immunogen :

4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein

Specificity :

Tyrosine-phosphorylated proteins from all species.

Package Size :

100 μL

Uses :

Western Blotting;ELISA

Storage :

6 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.