This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
catalog :
16-193
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
biotin
antigen modification :
phosphorylated
clone name :
JBW301
reactivity :
human, mouse
application :
western blot, immunocytochemistry
citations: 323
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig s7a
  • immunocytochemistry; mouse; loading ...; fig 6d
Zhong J, Li X, Cai W, Wang Y, Dong S, Yang J, et al. TET1 modulates H4K16 acetylation by controlling auto-acetylation of hMOF to affect gene regulation and DNA repair function. Nucleic Acids Res. 2017;45:672-684 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 2
Cloutier J, Mahadevaiah S, Elinati E, Tóth A, Turner J. Mammalian meiotic silencing exhibits sexually dimorphic features. Chromosoma. 2016;125:215-26 pubmed publisher
  • western blot; human; fig 3a
Torres M, Pandita R, Kulak O, Kumar R, Formstecher E, Horikoshi N, et al. Role of the Exocyst Complex Component Sec6/8 in Genomic Stability. Mol Cell Biol. 2015;35:3633-45 pubmed publisher
Pefani D, Tognoli M, Pirincci Ercan D, Gorgoulis V, O Neill E. MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B. EMBO J. 2018;37: pubmed publisher
Cloutier J, Mahadevaiah S, Elinati E, Nussenzweig A, Tóth A, Turner J. Histone H2AFX Links Meiotic Chromosome Asynapsis to Prophase I Oocyte Loss in Mammals. PLoS Genet. 2015;11:e1005462 pubmed publisher
Moore Z, Chakrabarti G, Luo X, Ali A, Hu Z, Fattah F, et al. NAMPT inhibition sensitizes pancreatic adenocarcinoma cells to tumor-selective, PAR-independent metabolic catastrophe and cell death induced by β-lapachone. Cell Death Dis. 2015;6:e1599 pubmed publisher
Xiong X, Du Z, Wang Y, Feng Z, Fan P, Yan C, et al. 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. Nucleic Acids Res. 2015;43:1659-70 pubmed publisher
Meisenberg C, Gilbert D, Chalmers A, Haley V, Gollins S, Ward S, et al. Clinical and cellular roles for TDP1 and TOP1 in modulating colorectal cancer response to irinotecan. Mol Cancer Ther. 2015;14:575-85 pubmed publisher
Ciccia A, Huang J, Izhar L, Sowa M, Harper J, Elledge S. Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response. Proc Natl Acad Sci U S A. 2014;111:18631-6 pubmed publisher
Wit N, Buoninfante O, van den Berk P, Jansen J, Hogenbirk M, de Wind N, et al. Roles of PCNA ubiquitination and TLS polymerases κ and η in the bypass of methyl methanesulfonate-induced DNA damage. Nucleic Acids Res. 2015;43:282-94 pubmed publisher
Chene G, Cayre A, Raoelfils I, Lagarde N, Dauplat J, Penault Llorca F. Morphological and immunohistochemical pattern of tubo-ovarian dysplasia and serous tubal intraepithelial carcinoma. Eur J Obstet Gynecol Reprod Biol. 2014;183:89-95 pubmed publisher
Cui L, Tse K, Zahedi P, Harding S, Zafarana G, Jaffray D, et al. Hypoxia and cellular localization influence the radiosensitizing effect of gold nanoparticles (AuNPs) in breast cancer cells. Radiat Res. 2014;182:475-88 pubmed publisher
Morgado Palacin L, Llanos S, Urbano Cuadrado M, Blanco Aparicio C, Megias D, Pastor J, et al. Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway. Carcinogenesis. 2014;35:2822-30 pubmed publisher
Slaats G, Ghosh A, Falke L, Le Corre S, Shaltiël I, van de Hoek G, et al. Nephronophthisis-associated CEP164 regulates cell cycle progression, apoptosis and epithelial-to-mesenchymal transition. PLoS Genet. 2014;10:e1004594 pubmed publisher
Hu Y, Wang C, Huang K, Xia F, Parvin J, Mondal N. Regulation of 53BP1 protein stability by RNF8 and RNF168 is important for efficient DNA double-strand break repair. PLoS ONE. 2014;9:e110522 pubmed publisher
Li Z, Wang H, Wang Y, Murnane J, Dynan W. Effect of radiation quality on mutagenic joining of enzymatically-induced DNA double-strand breaks in previously irradiated human cells. Radiat Res. 2014;182:573-9 pubmed publisher
McQuade C, Al Zaki A, Desai Y, Vido M, Sakhuja T, Cheng Z, et al. A multifunctional nanoplatform for imaging, radiotherapy, and the prediction of therapeutic response. Small. 2015;11:834-43 pubmed publisher
Jackstadt R, Hermeking H. AP4 is required for mitogen- and c-MYC-induced cell cycle progression. Oncotarget. 2014;5:7316-27 pubmed
Sugahara R, Mon H, Lee J, Shiotsuki T, Kusakabe T. Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells. FEBS Lett. 2014;588:3959-63 pubmed publisher
Ishiguro K, Lin Z, Penketh P, Shyam K, Zhu R, Baumann R, et al. Distinct mechanisms of cell-kill by triapine and its terminally dimethylated derivative Dp44mT due to a loss or gain of activity of their copper(II) complexes. Biochem Pharmacol. 2014;91:312-22 pubmed publisher
Karnak D, Engelke C, Parsels L, Kausar T, Wei D, Robertson J, et al. Combined inhibition of Wee1 and PARP1/2 for radiosensitization in pancreatic cancer. Clin Cancer Res. 2014;20:5085-96 pubmed publisher
Dean R, Fam H, An J, Choi K, Shimizu Y, Jones S, et al. Identification of a putative Tdp1 inhibitor (CD00509) by in vitro and cell-based assays. J Biomol Screen. 2014;19:1372-82 pubmed publisher
Feng F, Speers C, Liu M, Jackson W, Moon D, Rinkinen J, et al. Targeted radiosensitization with PARP1 inhibition: optimization of therapy and identification of biomarkers of response in breast cancer. Breast Cancer Res Treat. 2014;147:81-94 pubmed publisher
Bartkova J, Hoei Hansen C, Krizova K, Hamerlik P, Skakkebæk N, Rajpert De Meyts E, et al. Patterns of DNA damage response in intracranial germ cell tumors versus glioblastomas reflect cell of origin rather than brain environment: implications for the anti-tumor barrier concept and treatment. Mol Oncol. 2014;8:1667-78 pubmed publisher
Park S, Kang J, Kim S, Kim H, Hong S, Lee Y, et al. Smad7 enhances ATM activity by facilitating the interaction between ATM and Mre11-Rad50-Nbs1 complex in DNA double-strand break repair. Cell Mol Life Sci. 2015;72:583-596 pubmed publisher
Jones R, Kotsantis P, Stewart G, Groth P, Petermann E. BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcitabine. Mol Cancer Ther. 2014;13:2412-21 pubmed publisher
Carlessi L, Fusar Poli E, Bechi G, Mantegazza M, Pascucci B, Narciso L, et al. Functional and molecular defects of hiPSC-derived neurons from patients with ATM deficiency. Cell Death Dis. 2014;5:e1342 pubmed publisher
Drutovic D, Chripkova M, Pilatova M, Kruzliak P, Perjesi P, Sarissky M, et al. Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells. Tumour Biol. 2014;35:9967-75 pubmed publisher
Znojek P, Willmore E, Curtin N. Preferential potentiation of topoisomerase I poison cytotoxicity by PARP inhibition in S phase. Br J Cancer. 2014;111:1319-26 pubmed publisher
Manguan García C, Pintado Berninches L, Carrillo J, Machado Pinilla R, Sastre L, Perez Quilis C, et al. Expression of the genetic suppressor element 24.2 (GSE24.2) decreases DNA damage and oxidative stress in X-linked dyskeratosis congenita cells. PLoS ONE. 2014;9:e101424 pubmed publisher
Anderson D, Mirzayans R, Andrais B, Siegbahn E, Fallone B, Warkentin B. Spatial and temporal distribution of ?H2AX fluorescence in human cell cultures following synchrotron-generated X-ray microbeams: lack of correlation between persistent ?H2AX foci and apoptosis. J Synchrotron Radiat. 2014;21:801-10 pubmed publisher
Bridges K, Toniatti C, Buser C, Liu H, Buchholz T, Meyn R. Niraparib (MK-4827), a novel poly(ADP-Ribose) polymerase inhibitor, radiosensitizes human lung and breast cancer cells. Oncotarget. 2014;5:5076-86 pubmed
Glover K, Markell L, Donner E, Han X. Protein kinase C-activating tumor promoters modulate the DNA damage response in UVC-irradiated TK6 cells. Toxicol Lett. 2014;229:210-9 pubmed publisher
Cervelli T, Panetta D, Navarra T, Andreassi M, Basta G, Galli A, et al. Effects of single and fractionated low-dose irradiation on vascular endothelial cells. Atherosclerosis. 2014;235:510-8 pubmed publisher
Gao C, Bourke E, Scobie M, Famme M, Koolmeister T, Helleday T, et al. Rational design and validation of a Tip60 histone acetyltransferase inhibitor. Sci Rep. 2014;4:5372 pubmed publisher
Trapp Fragnet L, Bencherit D, Chabanne Vautherot D, Le Vern Y, Remy S, Boutet Robinet E, et al. Cell cycle modulation by Marek's disease virus: the tegument protein VP22 triggers S-phase arrest and DNA damage in proliferating cells. PLoS ONE. 2014;9:e100004 pubmed publisher
Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, et al. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol. 2014;193:950-60 pubmed publisher
Samuni Y, Wink D, Krishna M, Mitchell J, Goldstein S. Suberoylanilide hydroxamic acid radiosensitizes tumor hypoxic cells in vitro through the oxidation of nitroxyl to nitric oxide. Free Radic Biol Med. 2014;73:291-8 pubmed publisher
Dinami R, Ercolani C, Petti E, Piazza S, Ciani Y, Sestito R, et al. miR-155 drives telomere fragility in human breast cancer by targeting TRF1. Cancer Res. 2014;74:4145-56 pubmed publisher
Yang A, Rajeshkumar N, Wang X, Yabuuchi S, Alexander B, Chu G, et al. Autophagy is critical for pancreatic tumor growth and progression in tumors with p53 alterations. Cancer Discov. 2014;4:905-13 pubmed publisher
Patterson M, Sutton R, Forrest I, Sharrock R, Lane M, Kaufmann A, et al. Assessing the function of homologous recombination DNA repair in malignant pleural effusion (MPE) samples. Br J Cancer. 2014;111:94-100 pubmed publisher
Mason J, Logan H, Budke B, Wu M, Pawlowski M, Weichselbaum R, et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res. 2014;74:3546-55 pubmed publisher
Vidi P, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, et al. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic Acids Res. 2014;42:6365-79 pubmed publisher
Mizukami H, Takahashi K, Inaba W, Tsuboi K, Osonoi S, Yoshida T, et al. Involvement of oxidative stress-induced DNA damage, endoplasmic reticulum stress, and autophagy deficits in the decline of ?-cell mass in Japanese type 2 diabetic patients. Diabetes Care. 2014;37:1966-74 pubmed publisher
Shahar O, Kalousi A, Eini L, Fisher B, Weiss A, Darr J, et al. A high-throughput chemical screen with FDA approved drugs reveals that the antihypertensive drug Spironolactone impairs cancer cell survival by inhibiting homology directed repair. Nucleic Acids Res. 2014;42:5689-701 pubmed publisher
Al Ejeh F, Staudacher A, Smyth D, Darby J, Denoyer D, Tsopelas C, et al. Postchemotherapy and tumor-selective targeting with the La-specific DAB4 monoclonal antibody relates to apoptotic cell clearance. J Nucl Med. 2014;55:772-9 pubmed publisher
Mohni K, Kavanaugh G, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res. 2014;74:2835-45 pubmed publisher
Chang H, Wang H, Chen C, Hung T, Hou M, Yuan S, et al. 5-azacytidine induces anoikis, inhibits mammosphere formation and reduces metalloproteinase 9 activity in MCF-7 human breast cancer cells. Molecules. 2014;19:3149-59 pubmed publisher
Münch S, Weidtkamp Peters S, Klement K, Grigaravicius P, Monajembashi S, Salomoni P, et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014;34:1733-46 pubmed publisher
Bokobza S, Jiang Y, Weber A, Devery A, Ryan A. Short-course treatment with gefitinib enhances curative potential of radiation therapy in a mouse model of human non-small cell lung cancer. Int J Radiat Oncol Biol Phys. 2014;88:947-54 pubmed publisher
Storm M, Kumpfmueller B, Bone H, Buchholz M, Sanchez Ripoll Y, Chaudhuri J, et al. Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells. PLoS ONE. 2014;9:e89821 pubmed publisher
Morales J, Richard P, Rommel A, Fattah F, Motea E, Patidar P, et al. Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair. Nucleic Acids Res. 2014;42:4996-5006 pubmed publisher
Ramaekers C, van den Beucken T, Bristow R, Chiu R, Durocher D, Wouters B. RNF8-independent Lys63 poly-ubiquitylation prevents genomic instability in response to replication-associated DNA damage. PLoS ONE. 2014;9:e89997 pubmed publisher
Hosono Y, Abe T, Higuchi M, Kajii K, Sakuraba S, Tada S, et al. Tipin functions in the protection against topoisomerase I inhibitor. J Biol Chem. 2014;289:11374-84 pubmed publisher
Köpper F, Binkowski A, Bierwirth C, Dobbelstein M. The MAPK-activated protein kinase 2 mediates gemcitabine sensitivity in pancreatic cancer cells. Cell Cycle. 2014;13:884-9 pubmed publisher
Iacobazzi D, Mangialardi G, Gubernator M, Hofner M, Wielscher M, Vierlinger K, et al. Increased antioxidant defense mechanism in human adventitia-derived progenitor cells is associated with therapeutic benefit in ischemia. Antioxid Redox Signal. 2014;21:1591-604 pubmed publisher
Berkenkamp B, Susnik N, Baisantry A, Kuznetsova I, Jacobi C, Sörensen Zender I, et al. In vivo and in vitro analysis of age-associated changes and somatic cellular senescence in renal epithelial cells. PLoS ONE. 2014;9:e88071 pubmed publisher
Petrova N, Velichko A, Kantidze O, Razin S. Heat shock-induced dissociation of TRF2 from telomeres does not initiate a telomere-dependent DNA damage response. Cell Biol Int. 2014;38:675-81 pubmed publisher
Islam M, Thomas S, Murty V, Sedoris K, Miller D. c-Myc quadruplex-forming sequence Pu-27 induces extensive damage in both telomeric and nontelomeric regions of DNA. J Biol Chem. 2014;289:8521-31 pubmed publisher
Belanger F, Rajotte V, Drobetsky E. A majority of human melanoma cell lines exhibits an S phase-specific defect in excision of UV-induced DNA photoproducts. PLoS ONE. 2014;9:e85294 pubmed publisher
Morita A, Tanimoto K, Murakami T, Morinaga T, Hosoi Y. Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells. Biochem Biophys Res Commun. 2014;443:1286-90 pubmed publisher
Khalaj M, Abbasi A, Yamanishi H, Akiyama K, Wakitani S, Kikuchi S, et al. A missense mutation in Rev7 disrupts formation of Pol?, impairing mouse development and repair of genotoxic agent-induced DNA lesions. J Biol Chem. 2014;289:3811-24 pubmed publisher
Ferlazzo M, Sonzogni L, Granzotto A, Bodgi L, Lartin O, Devic C, et al. Mutations of the Huntington's disease protein impact on the ATM-dependent signaling and repair pathways of the radiation-induced DNA double-strand breaks: corrective effect of statins and bisphosphonates. Mol Neurobiol. 2014;49:1200-11 pubmed publisher
Vitale N, Kisslinger A, Paladino S, Procaccini C, Matarese G, Pierantoni G, et al. Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. PLoS ONE. 2013;8:e80728 pubmed publisher
Frame F, Pellacani D, Collins A, Simms M, Mann V, Jones G, et al. HDAC inhibitor confers radiosensitivity to prostate stem-like cells. Br J Cancer. 2013;109:3023-33 pubmed publisher
Neumann J, Boerries M, Kohler R, Giaisi M, Krammer P, Busch H, et al. The natural anticancer compound rocaglamide selectively inhibits the G1-S-phase transition in cancer cells through the ATM/ATR-mediated Chk1/2 cell cycle checkpoints. Int J Cancer. 2014;134:1991-2002 pubmed publisher
Khongkow P, Karunarathna U, Khongkow M, Gong C, Gomes A, Yague E, et al. FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance. Oncogene. 2014;33:4144-55 pubmed publisher
Luo T, Cui S, Bian C, Yu X. Uhrf2 is important for DNA damage response in vascular smooth muscle cells. Biochem Biophys Res Commun. 2013;441:65-70 pubmed publisher
Secher T, Samba Louaka A, Oswald E, Nougayrede J. Escherichia coli producing colibactin triggers premature and transmissible senescence in mammalian cells. PLoS ONE. 2013;8:e77157 pubmed publisher
Helchowski C, Skow L, Roberts K, Chute C, Canman C. A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci. Cell Cycle. 2013;12:3749-58 pubmed publisher
Hu L, Filippakis H, Huang H, Yen T, Gjoerup O. Replication stress and mitotic dysfunction in cells expressing simian virus 40 large T antigen. J Virol. 2013;87:13179-92 pubmed publisher
Wu Y, Deighan C, Miller B, Balasubramanian P, Lustberg M, Zborowski M, et al. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods. 2013;64:169-82 pubmed publisher
Akiyama K, Noguchi J, Hirose M, Kajita S, Katayama K, Khalaj M, et al. A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice. J Biol Chem. 2013;288:31830-41 pubmed publisher
Vassena L, Giuliani E, Matusali G, Cohen r, Doria M. The human immunodeficiency virus type 1 Vpr protein upregulates PVR via activation of the ATR-mediated DNA damage response pathway. J Gen Virol. 2013;94:2664-9 pubmed publisher
Men N, Kikuchi K, Nakai M, Fukuda A, Tanihara F, Noguchi J, et al. Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection. Theriogenology. 2013;80:1033-44 pubmed publisher
Storci G, Bertoni S, De Carolis S, Papi A, Nati M, Ceccarelli C, et al. Slug/?-catenin-dependent proinflammatory phenotype in hypoxic breast cancer stem cells. Am J Pathol. 2013;183:1688-1697 pubmed publisher
Tu W, Li B, Huang B, Wang Y, Liu X, Guan H, et al. ?H2AX foci formation in the absence of DNA damage: mitotic H2AX phosphorylation is mediated by the DNA-PKcs/CHK2 pathway. FEBS Lett. 2013;587:3437-43 pubmed publisher
Iwasa H, Kudo T, Maimaiti S, Ikeda M, Maruyama J, Nakagawa K, et al. The RASSF6 tumor suppressor protein regulates apoptosis and the cell cycle via MDM2 protein and p53 protein. J Biol Chem. 2013;288:30320-9 pubmed publisher
Di Francesco A, De Pittà C, Moret F, Barbieri V, Celotti L, Mognato M. The DNA-damage response to ?-radiation is affected by miR-27a in A549 cells. Int J Mol Sci. 2013;14:17881-96 pubmed publisher
Yu L, Zhao Y, Quan C, Ji W, Zhu J, Huang Y, et al. Gemcitabine eliminates double minute chromosomes from human ovarian cancer cells. PLoS ONE. 2013;8:e71988 pubmed publisher
Chen Y, Kamili A, Hardy J, Groblewski G, Khanna K, Byrne J. Tumor protein D52 represents a negative regulator of ATM protein levels. Cell Cycle. 2013;12:3083-97 pubmed publisher
Lawrence M, Ormsby R, Blyth B, Bezak E, England G, Newman M, et al. Lack of high-dose radiation mediated prostate cancer promotion and low-dose radiation adaptive response in the TRAMP mouse model. Radiat Res. 2013;180:376-88 pubmed publisher
Cornelissen B, Waller A, Able S, Vallis K. Molecular radiotherapy using cleavable radioimmunoconjugates that target EGFR and ?H2AX. Mol Cancer Ther. 2013;12:2472-82 pubmed publisher
Lai X, Egan L. Suppression of radiation-induced DNA double-strand break repair by MyD88 is accompanied by apoptosis and crypt loss in mouse colon. Oncogenesis. 2013;2:e62 pubmed publisher
Postel Vinay S, Bajrami I, Friboulet L, Elliott R, Fontebasso Y, Dorvault N, et al. A high-throughput screen identifies PARP1/2 inhibitors as a potential therapy for ERCC1-deficient non-small cell lung cancer. Oncogene. 2013;32:5377-87 pubmed publisher
Orim F, Bychkov A, Shimamura M, Nakashima M, Ito M, Matsuse M, et al. Thyrotropin signaling confers more aggressive features with higher genomic instability on BRAF(V600E)-induced thyroid tumors in a mouse model. Thyroid. 2014;24:502-10 pubmed publisher
Butin Israeli V, Adam S, Goldman R. Regulation of nucleotide excision repair by nuclear lamin b1. PLoS ONE. 2013;8:e69169 pubmed publisher
Wells C, Bhaskara S, Stengel K, Zhao Y, Sirbu B, Chagot B, et al. Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PLoS ONE. 2013;8:e68915 pubmed publisher
Bee L, Fabris S, Cherubini R, Mognato M, Celotti L. The efficiency of homologous recombination and non-homologous end joining systems in repairing double-strand breaks during cell cycle progression. PLoS ONE. 2013;8:e69061 pubmed publisher
Lin T, Ibrahim W, Peng C, Finegold M, Tsai R. A novel role of nucleostemin in maintaining the genome integrity of dividing hepatocytes during mouse liver development and regeneration. Hepatology. 2013;58:2176-87 pubmed publisher
Del Turco S, Ciofani G, Cappello V, Gemmi M, Cervelli T, Saponaro C, et al. Cytocompatibility evaluation of glycol-chitosan coated boron nitride nanotubes in human endothelial cells. Colloids Surf B Biointerfaces. 2013;111:142-9 pubmed publisher
Streppel M, Pai S, Campbell N, Hu C, Yabuuchi S, Canto M, et al. MicroRNA 223 is upregulated in the multistep progression of Barrett's esophagus and modulates sensitivity to chemotherapy by targeting PARP1. Clin Cancer Res. 2013;19:4067-78 pubmed publisher
Somaiah N, Yarnold J, Lagerqvist A, Rothkamm K, Helleday T. Homologous recombination mediates cellular resistance and fraction size sensitivity to radiation therapy. Radiother Oncol. 2013;108:155-61 pubmed publisher
Millet P, Granotier C, Etienne O, Boussin F. Radiation-induced upregulation of telomerase activity escapes PI3-kinase inhibition in two malignant glioma cell lines. Int J Oncol. 2013;43:375-82 pubmed publisher
Li Z, Hudson F, Wang H, Wang Y, Bian Z, Murnane J, et al. Increased mutagenic joining of enzymatically-induced DNA double-strand breaks in high-charge and energy particle irradiated human cells. Radiat Res. 2013;180:17-24 pubmed publisher
Li B, Shang Z, Yin J, Xu Q, Liu X, Wang Y, et al. PIG3 functions in DNA damage response through regulating DNA-PKcs homeostasis. Int J Biol Sci. 2013;9:425-34 pubmed publisher
Ulukaya E, Frame F, Cevatemre B, Pellacani D, Walker H, Mann V, et al. Differential cytotoxic activity of a novel palladium-based compound on prostate cell lines, primary prostate epithelial cells and prostate stem cells. PLoS ONE. 2013;8:e64278 pubmed publisher
Mahen R, Hattori H, Lee M, Sharma P, Jeyasekharan A, Venkitaraman A. A-type lamins maintain the positional stability of DNA damage repair foci in mammalian nuclei. PLoS ONE. 2013;8:e61893 pubmed publisher
Ryu S, Kawamura R, Naka R, Silberberg Y, Nakamura N, Nakamura C. Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA. J Biosci Bioeng. 2013;116:391-6 pubmed publisher
Muller I, Merk B, Voss K, Averbeck N, Jakob B, Durante M, et al. Species conserved DNA damage response at the inactive human X chromosome. Mutat Res. 2013;756:30-6 pubmed publisher
Kopova I, Bacakova L, Lavrentiev V, Vacik J. Growth and potential damage of human bone-derived cells on fresh and aged fullerene c60 films. Int J Mol Sci. 2013;14:9182-204 pubmed publisher
Meyer B, Voss K, Tobias F, Jakob B, Durante M, Taucher Scholz G. Clustered DNA damage induces pan-nuclear H2AX phosphorylation mediated by ATM and DNA-PK. Nucleic Acids Res. 2013;41:6109-18 pubmed publisher
Sorokina S, Markova E, Gursky J, Dobrovodsky J, Belyaev I. Relative biological efficiency of protons at low and therapeutic doses in induction of 53BP1/?H2AX foci in lymphocytes from umbilical cord blood. Int J Radiat Biol. 2013;89:716-23 pubmed publisher
Lu X, Parvathaneni S, Hara T, Lal A, Sharma S. Replication stress induces specific enrichment of RECQ1 at common fragile sites FRA3B and FRA16D. Mol Cancer. 2013;12:29 pubmed publisher
Chene G, Tchirkov A, Pierre Eymard E, Dauplat J, Raoelfils I, Cayre A, et al. Early telomere shortening and genomic instability in tubo-ovarian preneoplastic lesions. Clin Cancer Res. 2013;19:2873-82 pubmed publisher
Bahassi E, Li Y, Wise Draper T, Deng L, Wang J, Darnell C, et al. A patient-derived somatic mutation in the epidermal growth factor receptor ligand-binding domain confers increased sensitivity to cetuximab in head and neck cancer. Eur J Cancer. 2013;49:2345-55 pubmed publisher
Saha A, Kappes F, Mundade A, Deutzmann A, Rosmarin D, Legendre M, et al. Intercellular trafficking of the nuclear oncoprotein DEK. Proc Natl Acad Sci U S A. 2013;110:6847-52 pubmed publisher
Min A, Im S, Yoon Y, Song S, Nam H, Hur H, et al. RAD51C-deficient cancer cells are highly sensitive to the PARP inhibitor olaparib. Mol Cancer Ther. 2013;12:865-77 pubmed publisher
Berchtold C, Coughlin A, Kasper Z, Thibeault S. Paracrine potential of fibroblasts exposed to cigarette smoke extract with vascular growth factor induction. Laryngoscope. 2013;123:2228-36 pubmed publisher
Jelveh S, Kaspler P, Bhogal N, Mahmood J, Lindsay P, Okunieff P, et al. Investigations of antioxidant-mediated protection and mitigation of radiation-induced DNA damage and lipid peroxidation in murine skin. Int J Radiat Biol. 2013;89:618-27 pubmed publisher
Jha H, Upadhyay S, A J Prasad M, Lu J, Cai Q, Saha A, et al. H2AX phosphorylation is important for LANA-mediated Kaposi's sarcoma-associated herpesvirus episome persistence. J Virol. 2013;87:5255-69 pubmed publisher
Ma H, Pederson T. The nucleolus stress response is coupled to an ATR-Chk1-mediated G2 arrest. Mol Biol Cell. 2013;24:1334-42 pubmed publisher
Lawrence M, Blyth B, Ormsby R, Tilley W, Sykes P. False-positive TUNEL staining observed in SV40 based transgenic murine prostate cancer models. Transgenic Res. 2013;22:1037-47 pubmed publisher
Okamoto K, Bartocci C, Ouzounov I, Diedrich J, Yates J, Denchi E. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature. 2013;494:502-5 pubmed publisher
Renaud E, Rosselli F. FANC pathway promotes UV-induced stalled replication forks recovery by acting both upstream and downstream Pol? and Rev1. PLoS ONE. 2013;8:e53693 pubmed publisher
Karan T, Moiseenko V, Gill B, Horwood R, Kyle A, Minchinton A. Radiobiological effects of altering dose rate in filter-free photon beams. Phys Med Biol. 2013;58:1075-82 pubmed publisher
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product information
Catalog Number :
16-193
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P16104
Host Name :
Mouse
Antigen :
phospho-Histone H2A.X (Ser139)
Clone :
JBW301
Conjugate :
Biotin
Isotype :
IgG1
Product Description :
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
Cross Reactivity :
Human
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
ALT Names :
H2AXS139P;Histone H2A.X (phospho S139);H2A histone family, member X;H2AX histone
Immunogen :
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Specificity :
Recognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
Package Size :
100 µg
Uses :
Immunocytochemistry;Western Blotting
Storage :
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
company information
EMD Millipore
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Billerica, Massachusetts 01821
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https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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