This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
catalog :
16-101
quantity :
1 mg
clonality :
monoclonal
host :
mouse
conjugate :
agarose
clone name :
4G10®
application :
immunoprecipitation
citations: 115
Reference
Illert A, Seitz A, Rummelt C, Kreutmair S, Engh R, Goodstal S, et al. Inhibition of Aurora kinase B is important for biologic activity of the dual inhibitors of BCR-ABL and Aurora kinases R763/AS703569 and PHA-739358 in BCR-ABL transformed cells. PLoS ONE. 2014;9:e112318 pubmed publisher
Gray E, Petsalaki E, James D, Bagshaw R, Stacey M, Rocks O, et al. Src homology 2 domain containing protein 5 (SH2D5) binds the breakpoint cluster region protein, BCR, and regulates levels of Rac1-GTP. J Biol Chem. 2014;289:35397-408 pubmed publisher
Cazet A, Charest J, Bennett D, Sambrooks C, Contessa J. Mannose phosphate isomerase regulates fibroblast growth factor receptor family signaling and glioma radiosensitivity. PLoS ONE. 2014;9:e110345 pubmed publisher
Lehnert C, Weiswange M, Jeremias I, Bayer C, Grunert M, Debatin K, et al. TRAIL-receptor costimulation inhibits proximal TCR signaling and suppresses human T cell activation and proliferation. J Immunol. 2014;193:4021-31 pubmed publisher
Mobasher M, de Toro Martín J, González Rodríguez Ã, Ramos S, Letzig L, James L, et al. Essential role of protein-tyrosine phosphatase 1B in the modulation of insulin signaling by acetaminophen in hepatocytes. J Biol Chem. 2014;289:29406-19 pubmed publisher
Osinalde N, Sánchez Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I. Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics. 2015;15:520-31 pubmed publisher
Jahn S, LAW M, Corsino P, Davis B, Harrison J, Law B. Signaling mechanisms that suppress the cytostatic actions of rapamycin. PLoS ONE. 2014;9:e99927 pubmed publisher
Tao W, Leng X, Chakraborty S, Ma H, Arlinghaus R. c-Abl activates janus kinase 2 in normal hematopoietic cells. J Biol Chem. 2014;289:21463-72 pubmed publisher
Newburn E, Duchemin A, Neff N, Hadjiconstantinou M. GM1 ganglioside enhances Ret signaling in striatum. J Neurochem. 2014;130:541-54 pubmed publisher
Tarafdar S, Poe J, Smithgall T. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28 pubmed publisher
Okada T, Nitta T, Kaji K, Takashima A, Oda H, Tamehiro N, et al. Differential function of Themis CABIT domains during T cell development. PLoS ONE. 2014;9:e89115 pubmed publisher
Keegan K, Li C, Li Z, Ma J, Ragains M, Coberly S, et al. Preclinical evaluation of AMG 925, a FLT3/CDK4 dual kinase inhibitor for treating acute myeloid leukemia. Mol Cancer Ther. 2014;13:880-9 pubmed publisher
Verdeguer F, Blättler S, Cunningham J, Hall J, Chim H, Puigserver P. Decreased genetic dosage of hepatic Yin Yang 1 causes diabetic-like symptoms. Mol Endocrinol. 2014;28:308-16 pubmed publisher
Nagao T, Kurosu T, Umezawa Y, Nogami A, Oshikawa G, Tohda S, et al. Proliferation and survival signaling from both Jak2-V617F and Lyn involving GSK3 and mTOR/p70S6K/4EBP1 in PVTL-1 cell line newly established from acute myeloid leukemia transformed from polycythemia vera. PLoS ONE. 2014;9:e84746 pubmed publisher
Basu S, Rajakaruna S, De Arcangelis A, Zhang L, Georges Labouesse E, Menko A. ?6 integrin transactivates insulin-like growth factor receptor-1 (IGF-1R) to regulate caspase-3-mediated lens epithelial cell differentiation initiation. J Biol Chem. 2014;289:3842-55 pubmed publisher
Stebbing R, Irvin S, Rivera Serrano E, Boehme K, Ikizler M, Yoder J, et al. An ITAM in a nonenveloped virus regulates activation of NF-?B, induction of beta interferon, and viral spread. J Virol. 2014;88:2572-83 pubmed publisher
Chaturvedi S, Dell E, Siegel D, Brittingham G, Seetharam S. Development of a rapid streptavidin capture-based assay for the tyrosine phosphorylated CSF-1R in peripheral blood mononuclear cells. Int J Biol Sci. 2013;9:1099-107 pubmed publisher
Kurosu T, Nagao T, Wu N, Oshikawa G, Miura O. Inhibition of the PI3K/Akt/GSK3 pathway downstream of BCR/ABL, Jak2-V617F, or FLT3-ITD downregulates DNA damage-induced Chk1 activation as well as G2/M arrest and prominently enhances induction of apoptosis. PLoS ONE. 2013;8:e79478 pubmed publisher
Luis B, Carpino N. Insights into the suppressor of T-cell receptor (TCR) signaling-1 (Sts-1)-mediated regulation of TCR signaling through the use of novel substrate-trapping Sts-1 phosphatase variants. FEBS J. 2014;281:696-707 pubmed publisher
Sanderson T, Mahapatra G, Pecina P, Ji Q, Yu K, Sinkler C, et al. Cytochrome C is tyrosine 97 phosphorylated by neuroprotective insulin treatment. PLoS ONE. 2013;8:e78627 pubmed publisher
Cho J, Kim H, Kim K, Yang D, Surh C, Sprent J. Unique features of naive CD8+ T cell activation by IL-2. J Immunol. 2013;191:5559-73 pubmed publisher
Thapa N, Choi S, Hedman A, Tan X, Anderson R. Phosphatidylinositol phosphate 5-kinase I?i2 in association with Src controls anchorage-independent growth of tumor cells. J Biol Chem. 2013;288:34707-18 pubmed publisher
Mukhopadhyay C, Zhao X, Maroni D, Band V, Naramura M. Distinct effects of EGFR ligands on human mammary epithelial cell differentiation. PLoS ONE. 2013;8:e75907 pubmed publisher
Diao R, Fok K, Zhao L, Chen H, Tang H, Chen J, et al. Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men. Reproduction. 2013;146:637-45 pubmed publisher
Sollome J, Thavathiru E, Camenisch T, Vaillancourt R. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal. 2014;26:70-82 pubmed publisher
Fekete A, Bogel G, Pesti S, Péterfi Z, Geiszt M, Buday L. EGF regulates tyrosine phosphorylation and membrane-translocation of the scaffold protein Tks5. J Mol Signal. 2013;8:8 pubmed publisher
Barboza L, Salmen S, Terán Ángel G, Peterson D, Berrueta L. A deficient translocation of CD3?, ZAP-70 and Grb2 to lipid raft, as a hallmark of defective adaptive immune response during chronic hepatitis B infection. Cell Immunol. 2013;284:9-19 pubmed publisher
Andrianifahanana M, Wilkes M, Gupta S, Rahimi R, Repellin C, Edens M, et al. Profibrotic TGF? responses require the cooperative action of PDGF and ErbB receptor tyrosine kinases. FASEB J. 2013;27:4444-54 pubmed publisher
Vockel M, Vestweber D. How T cells trigger the dissociation of the endothelial receptor phosphatase VE-PTP from VE-cadherin. Blood. 2013;122:2512-22 pubmed publisher
Rollet Labelle E, Vaillancourt M, Marois L, Newkirk M, Poubelle P, Naccache P. Cross-linking of IgGs bound on circulating neutrophils leads to an activation of endothelial cells: possible role of rheumatoid factors in rheumatoid arthritis-associated vascular dysfunction. J Inflamm (Lond). 2013;10:27 pubmed publisher
Vohra P, Thompson M, Sathish V, Kiel A, Jerde C, Pabelick C, et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta. 2013;1833:2953-2960 pubmed publisher
Hartung A, Ordelheide A, Staiger H, Melzer M, Haring H, Lammers R. The Akt substrate Girdin is a regulator of insulin signaling in myoblast cells. Biochim Biophys Acta. 2013;1833:2803-2811 pubmed publisher
Ames H, Wang A, Coughran A, Evaul K, Huang S, Graves C, et al. Huntingtin-interacting protein 1 phosphorylation by receptor tyrosine kinases. Mol Cell Biol. 2013;33:3580-93 pubmed publisher
Tong J, Sydorskyy Y, St Germain J, Taylor P, Tsao M, Moran M. Odin (ANKS1A) modulates EGF receptor recycling and stability. PLoS ONE. 2013;8:e64817 pubmed publisher
Nakajima C, Kulik A, Frotscher M, Herz J, Schafer M, Bock H, et al. Low density lipoprotein receptor-related protein 1 (LRP1) modulates N-methyl-D-aspartate (NMDA) receptor-dependent intracellular signaling and NMDA-induced regulation of postsynaptic protein complexes. J Biol Chem. 2013;288:21909-23 pubmed publisher
Mobasher M, Gonzalez Rodriguez A, Santamaria B, Ramos S, Martin M, Goya L, et al. Protein tyrosine phosphatase 1B modulates GSK3?/Nrf2 and IGFIR signaling pathways in acetaminophen-induced hepatotoxicity. Cell Death Dis. 2013;4:e626 pubmed publisher
Municio C, Alvarez Y, Montero O, Hugo E, Rodriguez M, Domingo E, et al. The response of human macrophages to ?-glucans depends on the inflammatory milieu. PLoS ONE. 2013;8:e62016 pubmed publisher
Zimman A, Berliner J, Graeber T. Phosphoproteomic analysis of aortic endothelial cells activated by oxidized phospholipids. Methods Mol Biol. 2013;1000:53-69 pubmed publisher
Sedek M, Strous G. SUMOylation is a regulator of the translocation of Jak2 between nucleus and cytosol. Biochem J. 2013;453:231-9 pubmed publisher
Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez Riaño A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
Du E, Li H, Jin S, Hu X, Qiu M, Han R. Evidence that TMEM67 causes polycystic kidney disease through activation of JNK/ERK-dependent pathways. Cell Biol Int. 2013;37:694-702 pubmed publisher
Ma Y, Lovekamp Swan T, Bekele W, Dohi A, Schreihofer D. Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats. Endocrinology. 2013;154:1589-97 pubmed publisher
O Leary E, Mazurkiewicz Munoz A, Argetsinger L, Maures T, Huynh H, Carter Su C. Identification of steroid-sensitive gene-1/Ccdc80 as a JAK2-binding protein. Mol Endocrinol. 2013;27:619-34 pubmed publisher
Luechapanichkul R, Chen X, Taha H, Vyas S, Guan X, Freitas M, et al. Specificity profiling of dual specificity phosphatase vaccinia VH1-related (VHR) reveals two distinct substrate binding modes. J Biol Chem. 2013;288:6498-510 pubmed publisher
Wu N, Kurosu T, Oshikawa G, Nagao T, Miura O. PECAM-1 is involved in BCR/ABL signaling and may downregulate imatinib-induced apoptosis of Philadelphia chromosome-positive leukemia cells. Int J Oncol. 2013;42:419-28 pubmed publisher
Kortum R, Rouquette Jazdanian A, Miyaji M, Merrill R, Markegard E, Pinski J, et al. A phospholipase C-γ1-independent, RasGRP1-ERK-dependent pathway drives lymphoproliferative disease in linker for activation of T cells-Y136F mutant mice. J Immunol. 2013;190:147-58 pubmed publisher
Shimamura S, Sasaki K, Tanaka M. The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins. J Biol Chem. 2013;288:1171-83 pubmed publisher
Gonzalez Navarro H, Vinue A, Sanz M, Delgado M, Pozo M, Serrano M, et al. Increased dosage of Ink4/Arf protects against glucose intolerance and insulin resistance associated with aging. Aging Cell. 2013;12:102-11 pubmed publisher
Hirve N, Levytskyy R, Rigaud S, Guimond D, Zal T, Sauer K, et al. A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions. PLoS ONE. 2012;7:e45158 pubmed publisher
Bogel G, Gujdár A, Geiszt M, Lanyi A, Fekete A, Sipeki S, et al. Frank-ter Haar syndrome protein Tks4 regulates epidermal growth factor-dependent cell migration. J Biol Chem. 2012;287:31321-9 pubmed publisher
Zhou C, Baltz J. JAK2 mediates the acute response to decreased cell volume in mouse preimplantation embryos by activating NHE1. J Cell Physiol. 2013;228:428-38 pubmed publisher
Crose L, Etheridge K, Chen C, Belyea B, Talbot L, Bentley R, et al. FGFR4 blockade exerts distinct antitumorigenic effects in human embryonal versus alveolar rhabdomyosarcoma. Clin Cancer Res. 2012;18:3780-90 pubmed publisher
Shimizu T, Ueda J, Ho J, Iwasaki K, Poellinger L, Harada I, et al. Dual inhibition of Src and GSK3 maintains mouse embryonic stem cells, whose differentiation is mechanically regulated by Src signaling. Stem Cells. 2012;30:1394-404 pubmed publisher
Law M, Corsino P, Jahn S, Davis B, Chen S, Patel B, et al. Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms. Oncogene. 2013;32:1316-29 pubmed publisher
Lahlou H, Sanguin Gendreau V, Frame M, Muller W. Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo. Breast Cancer Res. 2012;14:R36 pubmed
Palsgaard J, Emanuelli B, Winnay J, Sumara G, Karsenty G, Kahn C. Cross-talk between insulin and Wnt signaling in preadipocytes: role of Wnt co-receptor low density lipoprotein receptor-related protein-5 (LRP5). J Biol Chem. 2012;287:12016-26 pubmed publisher
Leow C, Coffman K, Inigo I, Breen S, Czapiga M, Soukharev S, et al. MEDI3617, a human anti-angiopoietin 2 monoclonal antibody, inhibits angiogenesis and tumor growth in human tumor xenograft models. Int J Oncol. 2012;40:1321-30 pubmed publisher
Min J, Reznichenko M, Poythress R, Gallant C, Vetterkind S, Li Y, et al. Src modulates contractile vascular smooth muscle function via regulation of focal adhesions. J Cell Physiol. 2012;227:3585-92 pubmed publisher
Yu P, Babicky M, Jaquish D, French R, Marayuma K, Mose E, et al. The RON-receptor regulates pancreatic cancer cell migration through phosphorylation-dependent breakdown of the hemidesmosome. Int J Cancer. 2012;131:1744-54 pubmed publisher
Gonzalez Rodriguez A, Mas Gutierrez J, Mirasierra M, Fernandez Perez A, Lee Y, Ko H, et al. Essential role of protein tyrosine phosphatase 1B in obesity-induced inflammation and peripheral insulin resistance during aging. Aging Cell. 2012;11:284-96 pubmed publisher
Myers M, Manning H, Coffey R, Liebler D. Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling. Mol Cell Proteomics. 2012;11:M111.015222 pubmed publisher
Nagao T, Oshikawa G, Wu N, Kurosu T, Miura O. DNA damage stress and inhibition of Jak2-V617F cause its degradation and synergistically induce apoptosis through activation of GSK3?. PLoS ONE. 2011;6:e27397 pubmed publisher
Cesca F, Yabe A, Spencer Dene B, Scholz Starke J, Medrihan L, Maden C, et al. Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems. Cell Death Differ. 2012;19:194-208 pubmed publisher
Bromberg White J, Boguslawski E, Hekman D, Kort E, Duesbery N. Persistent inhibition of oxygen-induced retinal neovascularization by anthrax lethal toxin. Invest Ophthalmol Vis Sci. 2011;52:8979-92 pubmed publisher
Pötz O, Schneiderhan Marra N, Henzler T, Herget T, Joos T. Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays. Methods Mol Biol. 2012;795:191-202 pubmed publisher
Zaika E, Wei J, Yin D, Andl C, Moll U, El Rifai W, et al. p73 protein regulates DNA damage repair. FASEB J. 2011;25:4406-14 pubmed publisher
Hale C, Dietz K, Varela J, Wood C, Zirlin B, Leverich L, et al. Essential role for vav Guanine nucleotide exchange factors in brain-derived neurotrophic factor-induced dendritic spine growth and synapse plasticity. J Neurosci. 2011;31:12426-36 pubmed publisher
Balgi A, Diering G, Donohue E, Lam K, Fonseca B, Zimmerman C, et al. Regulation of mTORC1 signaling by pH. PLoS ONE. 2011;6:e21549 pubmed publisher
McNulty S, Powell K, Erneux C, Kalman D. The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus. J Virol. 2011;85:7402-10 pubmed publisher
Jung A, Kaushansky A, MacBeath G, Kaushansky K. Tensin2 is a novel mediator in thrombopoietin (TPO)-induced cellular proliferation by promoting Akt signaling. Cell Cycle. 2011;10:1838-44 pubmed
Curtiss M, Hostager B, Stepniak E, Singh M, Manhica N, Knisz J, et al. Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). Mol Immunol. 2011;48:1424-31 pubmed publisher
Helmer R, Dertien J, Chilton B. Prolactin induces Jak2 phosphorylation of RUSHY195. Mol Cell Endocrinol. 2011;338:79-83 pubmed publisher
San Luis B, Sondgeroth B, Nassar N, Carpino N. Sts-2 is a phosphatase that negatively regulates zeta-associated protein (ZAP)-70 and T cell receptor signaling pathways. J Biol Chem. 2011;286:15943-54 pubmed publisher
Roy R, Moses M. ADAM12 induces estrogen-independence in breast cancer cells. Breast Cancer Res Treat. 2012;131:731-41 pubmed publisher
Raven J, Williams V, Wang S, Tremblay M, Muller W, Durbin J, et al. Stat1 is a suppressor of ErbB2/Neu-mediated cellular transformation and mouse mammary gland tumor formation. Cell Cycle. 2011;10:794-804 pubmed
So T, Choi H, Croft M. OX40 complexes with phosphoinositide 3-kinase and protein kinase B (PKB) to augment TCR-dependent PKB signaling. J Immunol. 2011;186:3547-55 pubmed publisher
Peterson L, Shaw L, Joetham A, Sakaguchi S, Gelfand E, Dragone L. SLAP deficiency enhances number and function of regulatory T cells preventing chronic autoimmune arthritis in SKG mice. J Immunol. 2011;186:2273-81 pubmed publisher
Klaus Heisen D, Nurisso A, Pietraszewska Bogiel A, Mbengue M, Camut S, Timmers T, et al. Structure-function similarities between a plant receptor-like kinase and the human interleukin-1 receptor-associated kinase-4. J Biol Chem. 2011;286:11202-10 pubmed publisher
Brockmeyer C, Paster W, Pepper D, Tan C, Trudgian D, McGowan S, et al. T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component. J Biol Chem. 2011;286:7535-47 pubmed publisher
Chao J, Gui P, Zamponi G, Davis G, Davis M. Spatial association of the Cav1.2 calcium channel with ?5?1-integrin. Am J Physiol Cell Physiol. 2011;300:C477-89 pubmed publisher
Driessens G, Zheng Y, Locke F, Cannon J, Gounari F, Gajewski T. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-?1 phosphorylation. J Immunol. 2011;186:784-90 pubmed publisher
Modi H, Li L, Chu S, Rossi J, Yee J, Bhatia R. Inhibition of Grb2 expression demonstrates an important role in BCR-ABL-mediated MAPK activation and transformation of primary human hematopoietic cells. Leukemia. 2011;25:305-12 pubmed publisher
von Bubnoff N, Gorantla S, Engh R, Oliveira T, Thöne S, Aberg E, et al. The low frequency of clinical resistance to PDGFR inhibitors in myeloid neoplasms with abnormalities of PDGFRA might be related to the limited repertoire of possible PDGFRA kinase domain mutations in vitro. Oncogene. 2011;30:933-43 pubmed publisher
Gianni D, Taulet N, DerMardirossian C, Bokoch G. c-Src-mediated phosphorylation of NoxA1 and Tks4 induces the reactive oxygen species (ROS)-dependent formation of functional invadopodia in human colon cancer cells. Mol Biol Cell. 2010;21:4287-98 pubmed publisher
Lin S, Gordon K, Kaplan N, Getsios S. Ligand targeting of EphA2 enhances keratinocyte adhesion and differentiation via desmoglein 1. Mol Biol Cell. 2010;21:3902-14 pubmed publisher
Judson M, Eagleson K, Wang L, Levitt P. Evidence of cell-nonautonomous changes in dendrite and dendritic spine morphology in the met-signaling-deficient mouse forebrain. J Comp Neurol. 2010;518:4463-78 pubmed publisher
Chen C, Krishnan R, Zhou E, Ramachandran A, Tambe D, Rajendran K, et al. Fluidization and resolidification of the human bladder smooth muscle cell in response to transient stretch. PLoS ONE. 2010;5:e12035 pubmed publisher
Tyner J, Fletcher L, Wang E, Yang W, Rutenberg Schoenberg M, Beadling C, et al. MET receptor sequence variants R970C and T992I lack transforming capacity. Cancer Res. 2010;70:6233-7 pubmed publisher
Helmer R, Panchoo M, Dertien J, Bhakta S, Hewetson A, Chilton B. Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH signaling. Mol Cell Endocrinol. 2010;325:143-9 pubmed publisher
Chagnon M, Wu C, Nakazawa T, Yamamoto T, Noda M, Blanchetot C, et al. Receptor tyrosine phosphatase sigma (RPTP?) regulates, p250GAP, a novel substrate that attenuates Rac signaling. Cell Signal. 2010;22:1626-33 pubmed publisher
Grasis J, Guimond D, Cam N, Herman K, Magotti P, Lambris J, et al. In vivo significance of ITK-SLP-76 interaction in cytokine production. Mol Cell Biol. 2010;30:3596-609 pubmed publisher
Hammond D, Hyde R, Kratchmarova I, Beynon R, Blagoev B, Clague M. Quantitative analysis of HGF and EGF-dependent phosphotyrosine signaling networks. J Proteome Res. 2010;9:2734-42 pubmed publisher
Sauer L, Gitenay D, Vo C, Baron V. Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells. Oncogene. 2010;29:2628-37 pubmed publisher
Gonzalez Rodriguez A, Mas Gutierrez J, Sanz González S, Ros M, Burks D, Valverde A. Inhibition of PTP1B restores IRS1-mediated hepatic insulin signaling in IRS2-deficient mice. Diabetes. 2010;59:588-99 pubmed publisher
Hsu S, Miller S, Wang Y, Hung M. Nuclear EGFR is required for cisplatin resistance and DNA repair. Am J Transl Res. 2009;1:249-58 pubmed
Carpino N, Chen Y, Nassar N, Oh H. The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways. Mol Immunol. 2009;46:3224-31 pubmed publisher
Jones M, Shawe Taylor A, Williams C, Poole A. Characterization of a novel focal adhesion kinase inhibitor in human platelets. Biochem Biophys Res Commun. 2009;389:198-203 pubmed publisher
Zarrinkar P, Gunawardane R, Cramer M, Gardner M, Brigham D, Belli B, et al. AC220 is a uniquely potent and selective inhibitor of FLT3 for the treatment of acute myeloid leukemia (AML). Blood. 2009;114:2984-92 pubmed publisher
Wang H, Wu J, Chia J, Yang C, Wu Y, Juang R. Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site. J Agric Food Chem. 2009;57:7348-55 pubmed publisher
Nalbant P, Chang Y, Birkenfeld J, Chang Z, Bokoch G. Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge. Mol Biol Cell. 2009;20:4070-82 pubmed publisher
Yano N, Suzuki D, Endoh M, Cao T, Dahdah J, Tseng A, et al. High ambient glucose induces angiotensin-independent AT-1 receptor activation, leading to increases in proliferation and extracellular matrix accumulation in MES-13 mesangial cells. Biochem J. 2009;423:129-43 pubmed publisher
Sherrard R, Dixon K, Bakouche J, Rodger J, Lemaigre Dubreuil Y, Mariani J. Differential expression of TrkB isoforms switches climbing fiber-Purkinje cell synaptogenesis to selective synapse elimination. Dev Neurobiol. 2009;69:647-62 pubmed publisher
Strauss G, Lindquist J, Arhel N, Felder E, Karl S, Haas T, et al. CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling. J Exp Med. 2009;206:1379-93 pubmed publisher
Kobayashi R, Patenia R, Ashizawa S, Vykoukal J. Targeted mass spectrometric analysis of N-terminally truncated isoforms generated via alternative translation initiation. FEBS Lett. 2009;583:2441-5 pubmed publisher
Marks D, Tucker K, Cavallin M, Mast T, Fadool D. Awake intranasal insulin delivery modifies protein complexes and alters memory, anxiety, and olfactory behaviors. J Neurosci. 2009;29:6734-51 pubmed publisher
Bhatnagar S, Damron H, Hillgartner F. Fibroblast growth factor-19, a novel factor that inhibits hepatic fatty acid synthesis. J Biol Chem. 2009;284:10023-33 pubmed publisher
Nelson E, Walker S, Kepich A, Gashin L, Hideshima T, Ikeda H, et al. Nifuroxazide inhibits survival of multiple myeloma cells by directly inhibiting STAT3. Blood. 2008;112:5095-102 pubmed publisher
Lee S, Feldman R, O Farrell P. An RNA interference screen identifies a novel regulator of target of rapamycin that mediates hypoxia suppression of translation in Drosophila S2 cells. Mol Biol Cell. 2008;19:4051-61 pubmed publisher
Nakamura N, Miranda Vizuete A, Miki K, Mori C, Eddy E. Cleavage of disulfide bonds in mouse spermatogenic cell-specific type 1 hexokinase isozyme is associated with increased hexokinase activity and initiation of sperm motility. Biol Reprod. 2008;79:537-45 pubmed publisher
Najy A, Day K, Day M. The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation. J Biol Chem. 2008;283:18393-401 pubmed publisher
Biswas S, Zhao Y, Nagalingam A, Gardner T, Sandirasegarane L. PDGF- and insulin/IGF-1-specific distinct modes of class IA PI 3-kinase activation in normal rat retinas and RGC-5 retinal ganglion cells. Invest Ophthalmol Vis Sci. 2008;49:3687-98 pubmed publisher
Voisin T, El Firar A, Rouyer Fessard C, Gratio V, Laburthe M. A hallmark of immunoreceptor, the tyrosine-based inhibitory motif ITIM, is present in the G protein-coupled receptor OX1R for orexins and drives apoptosis: a novel mechanism. FASEB J. 2008;22:1993-2002 pubmed publisher
Zhang J. The direct involvement of SirT1 in insulin-induced insulin receptor substrate-2 tyrosine phosphorylation. J Biol Chem. 2007;282:34356-64 pubmed
Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, et al. Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting. J Exp Med. 2007;204:1431-40 pubmed
Lee S, DUHE R. Kinase activity and subcellular distribution of a chimeric green fluorescent protein-tagged Janus kinase 2. J Biomed Sci. 2006;13:773-86 pubmed
product information
Catalog Number :
16-101
Subcategory :
Cell Signaling
Product Name :
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
Phosphotyrosine
Clone :
4G10®,
Conjugate :
Gel Immobilized
Product Description :
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
Cross Reactivity :
Vertebrates
Background :
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen :
Phosphotyramine-KLH.
Specificity :
Recognizes tyrosine-phosphorylated proteins from all species.
Package Size :
1 mg
Uses :
Immunoprecipitation
Storage :
Stable for 1 year at 2-8ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.