EED Antibody | EMD Millipore 09-774 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EED Antibody

catalog :

09-774

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dogs, chicken

application :

western blot

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 2a	Göllner S, Oellerich T, Agrawal Singh S, Schenk T, Klein H, Rohde C, et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med. 2017;23:69-78 pubmed publisher
western blot; mouse; fig s12c	Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher

product information

Catalog Number :

09-774

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-EED Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O75530

Host Name :

Rabbit

Antigen :

EED

Conjugate :

Purified

Product Description :

Anti-EED Antibody

Cross Reactivity :

Human;Mouse

Background :

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

ALT Names :

embryonic ectoderm development

Immunogen :

A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.

Specificity :

This antibody recognizes EED, Mr ~ 62-70 kDa.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.