REST Antibody | EMD Millipore 09-019 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

REST Antibody

catalog :

09-019

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4a	Lin T, Chang Y, Lee S, Campbell M, Wang T, Shen S, et al. REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling. Oncotarget. 2016;7:26137-51 pubmed publisher

product information

Catalog Number :

09-019

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-REST Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q13127

Host Name :

Rabbit

Antigen :

REST

Conjugate :

Affinity Purified

Product Description :

Anti-REST Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

REST (RE1-silencing transcription factor) also known as NRSF (neuron-restrictive silencer factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of REST to the NRSE represses neuronal gene transcription in non-neuronal cells. Although REST is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. REST contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. REST associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for REST outside the nervous system. Down-regulation of REST, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. REST is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

ALT Names :

Neural-restrictive silencer factor ;RE1-silencing transcription factor;X2 box repressor ;neuron restrictive silencer factor ;repressor binding to the X2 box

Immunogen :

GST fusion protein corresponding to residues 801-1097 of human REST.

Specificity :

Recognizes REST

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.