AS160 (Rab-GAP) Antibody | EMD Millipore 07-741 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

AS160 (Rab-GAP) Antibody

catalog :

07-741

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6d	Rittig N, Bach E, Thomsen H, Pedersen S, Nielsen T, Jørgensen J, et al. Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial. PLoS ONE. 2016;11:e0162167 pubmed publisher
western blot; rat; fig 2	Kawamoto E, Koshinaka K, Yoshimura T, Masuda H, Kawanaka K. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep. 2016;4: pubmed publisher
western blot; mouse; fig 5	Møller C, Kjøbsted R, Enriori P, Jensen T, Garcia Rudaz C, Litwak S, et al. ?-MSH Stimulates Glucose Uptake in Mouse Muscle and Phosphorylates Rab-GTPase-Activating Protein TBC1D1 Independently of AMPK. PLoS ONE. 2016;11:e0157027 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 1d	Vazirani R, Verma A, Sadacca L, Buckman M, Picatoste B, Beg M, et al. Disruption of Adipose Rab10-Dependent Insulin Signaling Causes Hepatic Insulin Resistance. Diabetes. 2016;65:1577-89 pubmed publisher
western blot; mouse; 1:1000	Dokas J, Chadt A, Joost H, Al Hasani H. Tbc1d1 deletion suppresses obesity in leptin-deficient mice. Int J Obes (Lond). 2016;40:1242-9 pubmed publisher
	Zhang Y, Xie L, Gunasekar S, Tong D, Mishra A, Gibson W, et al. SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis. Nat Cell Biol. 2017;19:504-517 pubmed publisher
	Blesson C, Chinnathambi V, Kumar S, Yallampalli C. Gestational Protein Restriction Impairs Glucose Disposal in the Gastrocnemius Muscles of Female Rats. Endocrinology. 2017;158:756-767 pubmed publisher
	Sakakibara I, Wurmser M, Dos Santos M, Santolini M, Ducommun S, Davaze R, et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle. 2016;6:30 pubmed publisher
	Bruno J, Brumfield A, Chaudhary N, Iaea D, McGraw T. SEC16A is a RAB10 effector required for insulin-stimulated GLUT4 trafficking in adipocytes. J Cell Biol. 2016;214:61-76 pubmed publisher
	Zhang W, Wu M, Kim T, Jariwala R, Garvey W, Luo N, et al. Skeletal Muscle TRIB3 Mediates Glucose Toxicity in Diabetes and High- Fat Diet-Induced Insulin Resistance. Diabetes. 2016;65:2380-91 pubmed publisher
	Tang Y, Wallace M, Sanchez Gurmaches J, Hsiao W, Li H, Lee P, et al. Adipose tissue mTORC2 regulates ChREBP-driven de novo lipogenesis and hepatic glucose metabolism. Nat Commun. 2016;7:11365 pubmed publisher

product information

Catalog Number :

07-741

Subcategory :

Cell Signaling

Product Name :

Anti-AS160 (Rab-GAP) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O60343

Host Name :

Rabbit

Antigen :

AS160 (Rab-GAP)

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-AS160 (Rab-GAP) Antibody

Cross Reactivity :

Human;Mouse;Rat;Canine

Background :

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

ALT Names :

Acrg embryonic lethality minimal region ortholog;Akt substrate of 160 kDa;TBC (Tre-2, BUB2, CDC16) domain-containing protein;TBC1 domain family, member

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.

Specificity :

Recognizes AS160 (Rab-GAP), Mr 160 kDa.

Package Size :

200 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.