MYPT1 | EMD Millipore 07-672 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MYPT1

catalog :

07-672

quantity :

200 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunoprecipitation, western blot knockout validation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:2500; fig s2a	Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher

product information

Catalog Number :

07-672

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-MYPT1

Product Type :

Antibodies

Clonality :

Polyclonal

Gene ID :

NP_002471

Host Name :

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-MYPT1

Cross Reactivity :

Human, Chicken, Vertebrates

Species Summary :

Human and chicken. Broad species cross-reactivity is expected due to sequence homology.

Background :

Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1.

ALT Names :

Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A

Immunogen :

MBP fusion protein corresponding to amino acids 714-1004 of chicken myosin phosphatase targeting subunit 1 (MYPT1).

Specificity :

Recognizes MYPT1, MW ~120-130 kDa.

Purity :

Protein A purfied

Package Size :

200 ug

Uses :

IP, WB

Storage :

Stable for 1 year at -20ºC from date of receipt.