Sp1 Antibody | EMD Millipore 07-645 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sp1 Antibody

catalog :

07-645

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, western blot knockout validation

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; loading ...; fig s1b	Gilmour J, O Connor L, Middleton C, Keane P, Gillemans N, Cazier J, et al. Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors. Epigenetics Chromatin. 2019;12:33 pubmed publisher
western blot; human; loading ...; fig 5a	Clementi E, Inglin L, Beebe E, Gsell C, Garajova Z, Markkanen E. Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction. BMC Biol. 2020;18:36 pubmed publisher
immunocytochemistry; mouse; fig s6d western blot; mouse; loading ...; fig s5e	Alim I, Caulfield J, Chen Y, Swarup V, Geschwind D, Ivanova E, et al. Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke. Cell. 2019;177:1262-1279.e25 pubmed publisher
western blot; mouse; loading ...; fig 2a	Nandi S, Mishra P. H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes. Sci Rep. 2017;7:3639 pubmed publisher
EMSA; human; loading ...; fig 5d	Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Hypermethylated LTR retrotransposon exhibits enhancer activity. Epigenetics. 2017;12:226-237 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 5f EMSA; mouse; loading ...; fig 5e western blot; mouse; 1:1000; loading ...; fig 5b	Yamamura K, Uruno T, Shiraishi A, Tanaka Y, Ushijima M, Nakahara T, et al. The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. Nat Commun. 2017;8:13946 pubmed publisher
EMSA; mouse; loading ...; fig 7c	McDonald E, Smith J, Cederberg R, White B. Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-B, specificity protein (SP)1-like and GATA-4 binding sites. Reprod Biol Endocrinol. 2016;14:36 pubmed publisher
chromatin immunoprecipitation; human; fig s5e	Haokip D, Goel I, Arya V, Sharma T, Kumari R, Priya R, et al. Transcriptional Regulation of Atp-Dependent Chromatin Remodeling Factors: Smarcal1 and Brg1 Mutually Co-Regulate Each Other. Sci Rep. 2016;6:20532 pubmed publisher
	Kar S, Shahshahan H, Hackfort B, Yadav S, Yadav R, Kambis T, et al. Exercise Training Promotes Cardiac Hydrogen Sulfide Biosynthesis and Mitigates Pyroptosis to Prevent High-Fat Diet-Induced Diabetic Cardiomyopathy. Antioxidants (Basel). 2019;8: pubmed publisher
	Dam E, Maier A, Hocking A, Carlin J, Buckner J. Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis. Front Immunol. 2018;9:1978 pubmed publisher
	Chow M, Gao L, Macmaniman J, Bicocca V, Chang B, Alumkal J, et al. Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL. Oncogene. 2018;37:5221-5232 pubmed publisher
	Hewett S, Shi J, Gong Y, Dhandapani K, Pilbeam C, Hewett J. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: OPPOSING ROLES FOR THE TRANSCRIPTION FACTORS CREB (cAMP RESPONSE ELEMENT BINDING) PROTEIN AND Sp1 (STIMULATORY PROTEIN-1). J Biol Chem. 2016;291:27279-27288 pubmed publisher
	Greene T, Tokuyama M, Knudsen G, Kunz M, Lin J, Greninger A, et al. A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression. elife. 2016;5: pubmed publisher
	Wein M, Liang Y, Göransson O, Sundberg T, Wang J, Williams E, et al. SIKs control osteocyte responses to parathyroid hormone. Nat Commun. 2016;7:13176 pubmed publisher
	Liu L, Ji P, Qu N, Pu W, Jiang D, Liu W, et al. The impact of high co-expression of Sp1 and HIF1? on prognosis of patients with hepatocellular cancer. Oncol Lett. 2016;12:504-512 pubmed
	Marek Bukowiec K, Aguado E, Miazek A. Phorbol ester-mediated re-expression of endogenous LAT adapter in J.CaM2 cells: a model for dissecting drivers and blockers of LAT transcription. Genes Immun. 2016;17:313-20 pubmed publisher
	Poletto M, Legrand A, Fletcher S, Dianov G. p53 coordinates base excision repair to prevent genomic instability. Nucleic Acids Res. 2016;44:3165-75 pubmed publisher

product information

Catalog Number :

07-645

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Sp1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P08047

Host Name :

Rabbit

Antigen :

Sp1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Sp1 Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

ALT Names :

Sp1 transcription factor;specificity protein 1

Immunogen :

Full length human Sp1 protein.

Specificity :

Recognizes Sp1, Mr 105 kDa and 95 kDa.

Package Size :

100 µg

Uses :

Electrophoretic Mobility Shift Assay;Immunofluorescence;Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.