Histone H2A.X Antibody | EMD Millipore 07-627 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone H2A.X Antibody

catalog :

07-627

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, chromatin immunoprecipitation

citations: 11

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 4f	Bleuyard J, Fournier M, Nakato R, Couturier A, Katou Y, Ralf C, et al. MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress. Proc Natl Acad Sci U S A. 2017;114:7671-7676 pubmed publisher
western blot; mouse; fig 4b	Marks D, Thomas R, Chin Y, Shah R, Khoo C, Benezra R. Mad2 Overexpression Uncovers a Critical Role for TRIP13 in Mitotic Exit. Cell Rep. 2017;19:1832-1845 pubmed publisher
western blot; human; loading ...; fig 1d	Leung J, Makharashvili N, Agarwal P, Chiu L, Pourpre R, Cammarata M, et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes Dev. 2017;31:260-274 pubmed publisher
chromatin immunoprecipitation; human; fig e10	Zhao D, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges F, et al. SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature. 2016;529:48-53 pubmed publisher
	Jones G, Rooney C, Griffin N, Roudier M, Young L, Garcia Trinidad A, et al. pRAD50: a novel and clinically applicable pharmacodynamic biomarker of both ATM and ATR inhibition identified using mass spectrometry and immunohistochemistry. Br J Cancer. 2018;119:1233-1243 pubmed publisher
	Yokoyama C, Baldridge M, Leung D, Zhao G, Desai C, Liu T, et al. LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens. J Biol Chem. 2018;293:6022-6038 pubmed publisher
	Feng Y, Xiang J, Liu S, Guo T, Yan G, Feng Y, et al. H2AX facilitates classical non-homologous end joining at the expense of limited nucleotide loss at repair junctions. Nucleic Acids Res. 2017;45:10614-10633 pubmed publisher
	Wheaton K, Campuzano D, Ma W, Sheinis M, Ho B, Brown G, et al. Progerin-Induced Replication Stress Facilitates Premature Senescence in Hutchinson-Gilford Progeria Syndrome. Mol Cell Biol. 2017;37: pubmed publisher
	Qiu Z, Zhang Z, Roschke A, Varga T, Aplan P. Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break. Sci Rep. 2017;7:43156 pubmed publisher
	Bhargava R, Carson C, Lee G, Stark J. Contribution of canonical nonhomologous end joining to chromosomal rearrangements is enhanced by ATM kinase deficiency. Proc Natl Acad Sci U S A. 2017;114:728-733 pubmed publisher
	Lima M, Bouzid H, Soares D, Selle F, Morel C, Galmarini C, et al. Dual inhibition of ATR and ATM potentiates the activity of trabectedin and lurbinectedin by perturbing the DNA damage response and homologous recombination repair. Oncotarget. 2016;7:25885-901 pubmed publisher

product information

Catalog Number :

07-627

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H2A.X Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P16104

Host Name :

Rabbit

Antigen :

Histone H2A.X

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-Histone H2A.X Antibody

Cross Reactivity :

Human

Background :

H2AX is involved in DNA repair and the maintenance of genomic stability. although its precise role is still to be defined. H2AX is phosphorylated soon after the appearance of double-stranded breaks. and has been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.

ALT Names :

H2A histone family, member X;H2AX histone

Immunogen :

KLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to amino acids 121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.

Specificity :

Recognizes histone H2A.X, Mr 15 kDa. A band at Mr 100 kDa was also observed.

Package Size :

200 µL

Uses :

Peptide Inhibition Assay;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.