REST Antibody | EMD Millipore 07-579 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

REST Antibody

catalog :

07-579

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, proximity ligation assay

citations: 4

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; human; loading ...; fig s4a western blot; human; loading ...; fig 4a	Lambert M, Terrone S, Giraud G, Benoit Pilven C, Cluet D, Combaret V, et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res. 2018;46:7686-7700 pubmed publisher
western blot; human; loading ...; fig 3b	Chang Y, Lin T, Campbell M, Pan C, Lee S, Lee H, et al. REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer. Sci Rep. 2017;7:42795 pubmed publisher
western blot; rat; 1:1000; fig 2 western blot; human; 1:1000; fig 2	Cui Y, Han J, Xiao Z, Chen T, Wang B, Chen B, et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep. 2016;6:23300 pubmed publisher
	Bennesch M, Segala G, Wider D, Picard D. LSD1 engages a corepressor complex for the activation of the estrogen receptor ? by estrogen and cAMP. Nucleic Acids Res. 2016;44:8655-8670 pubmed

product information

Catalog Number :

07-579

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-REST Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q13127

Host Name :

Rabbit

Antigen :

REST

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-REST Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

NRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells. Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system. Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

ALT Names :

Neural-restrictive silencer factor;RE1-silencing transcription factor;X2 box repressor;neuron restrictive silencer factor;repressor binding to the X2 box

Immunogen :

GST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).

Specificity :

Recognizes REST, Mr 200 kDa

Package Size :

200 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.