trimethyl-Histone H4 (Lys20) Antibody | EMD Millipore 07-463 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H4 (Lys20) Antibody

catalog :

07-463

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Entamoeba histolytica, human, rat, bovine

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; 1:250; fig 6	Mata Garrido J, Casafont I, Tapia O, Berciano M, Lafarga M. Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization. Acta Neuropathol Commun. 2016;4:41 pubmed publisher
western blot; bovine; 1:500; fig 2 western blot; Entamoeba histolytica; 1:500; fig 2	Lozano Amado D, Herrera Solorio A, ValdÃ©s J, AlemÃ¡n Lazarini L, Almaraz Barrera M, Luna Rivera E, et al. Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica. Parasit Vectors. 2016;9:19 pubmed publisher
	Kurup J, Kidder B. Identification of H4K20me3- and H3K4me3-associated RNAs using CARIP-Seq expands the transcriptional and epigenetic networks of embryonic stem cells. J Biol Chem. 2018;293:15120-15135 pubmed publisher
	Mata Garrido J, Tapia O, Casafont I, Berciano M, Cuadrado A, Lafarga M. Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA. Acta Neuropathol Commun. 2018;6:68 pubmed publisher
	Kidder B, He R, Wangsa D, Padilla Nash H, Bernardo M, Sheng S, et al. SMYD5 Controls Heterochromatin and Chromosome Integrity during Embryonic Stem Cell Differentiation. Cancer Res. 2017;77:6729-6745 pubmed publisher
	Kidder B, Hu G, Cui K, Zhao K. SMYD5 regulates H4K20me3-marked heterochromatin to safeguard ES cell self-renewal and prevent spurious differentiation. Epigenetics Chromatin. 2017;10:8 pubmed publisher

product information

Catalog Number :

07-463

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H4 (Lys20) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P62805

Host Name :

Rabbit

Antigen :

trimethyl-Histone H4 (Lys20)

Conjugate :

Affinity Purified

Product Description :

Anti-trimethyl-Histone H4 (Lys20) Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

ALT Names :

H4K20me3;Histone H4 (tri methyl K20);H4 histone family, member A;histone 1, H4a;histone cluster 1, H4a

Immunogen :

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.

Specificity :

This antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.

Package Size :

100 μg

Uses :

Immunoprecipitation;Western Blotting;Dot Blot;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt