dimethyl-Histone H3 (Lys27) Antibody | EMD Millipore 07-452 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Lys27) Antibody

catalog :

07-452

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; fig 4e	Lui J, Garrison P, Nguyen Q, Ad M, Keembiyehetty C, Chen W, et al. EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy. Nat Commun. 2016;7:13685 pubmed publisher
immunocytochemistry; fruit fly ; 1:500; fig 1d	Tirmarche S, Kimura S, Dubruille R, Horard B, Loppin B. Unlocking sperm chromatin at fertilization requires a dedicated egg thioredoxin in Drosophila. Nat Commun. 2016;7:13539 pubmed publisher
western blot; mouse; fig s6d	Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher
	Leadem B, Kagiampakis I, Wilson C, Cheung T, Arnott D, Trojer P, et al. A KDM5 Inhibitor Increases Global H3K4 Trimethylation Occupancy and Enhances the Biological Efficacy of 5-Aza-2'-Deoxycytidine. Cancer Res. 2018;78:1127-1139 pubmed publisher
	Song S, Jeon M, Nam J, Kang J, Lee Y, Kang J, et al. Aberrant GATA2 epigenetic dysregulation induces a GATA2/GATA6 switch in human gastric cancer. Oncogene. 2018;37:993-1004 pubmed publisher
	Lui J, Barnes K, Dong L, Yue S, Graber E, Rapaport R, et al. Ezh2 mutations found in the Weaver overgrowth syndrome cause a partial loss of H3K27 histone methyltransferase activity. J Clin Endocrinol Metab. 2017;: pubmed publisher
	Mursalimov S, Permyakova N, Deineko E, Houben A, Demidov D. Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes. Front Plant Sci. 2015;6:846 pubmed publisher
	Patel S, Bhumbra S, Paknikar R, Dressler G. Epigenetic mechanisms of Groucho/Grg/TLE mediated transcriptional repression. Mol Cell. 2012;45:185-95 pubmed publisher

product information

Catalog Number :

07-452

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Lys27) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Lys27)

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Lys27) Antibody

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K27me2;Histone H3 (di methyl K27)

Immunogen :

KLH-conjugated, synthetic 2X-branched peptide containing the sequence . AR me2 KSA. in which me2 K corresponds to dimethyl-lysine at residue 27 of human histone H3

Specificity :

Recognizes dimethylated histone H3 (Lys27).

Package Size :

200 μg

Uses :

Multiplexing;Cell Function Assay;Western Blotting;ChIP-seq;Dot Blot

Storage :

Stable for 2 years at 2-8°C from date of shipment.