monomethyl-Histone H3 (Lys9) Antibody | EMD Millipore 07-450 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

monomethyl-Histone H3 (Lys9) Antibody

catalog :

07-450

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Jayaram H, Hoelper D, Jain S, Cantone N, Lundgren S, Poy F, et al. S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3. Proc Natl Acad Sci U S A. 2016;113:6182-7 pubmed publisher
chromatin immunoprecipitation; Xenopus laevis; fig s2	Tamaoki K, Okada R, Ishihara A, Shiojiri N, Mochizuki K, Goda T, et al. Morphological, biochemical, transcriptional and epigenetic responses to fasting and refeeding in intestine of Xenopus laevis. Cell Biosci. 2016;6:2 pubmed publisher
	Peter C, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun. 2019;10:4112 pubmed publisher

product information

Catalog Number :

07-450

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-monomethyl-Histone H3 (Lys9) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

monomethyl-Histone H3 (Lys9)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-monomethyl-Histone H3 (Lys9) Antibody

Cross Reactivity :

Human;Mouse;Chicken

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K9me1;Histone H3 (mono methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKST… in which meK corresponds to monomethyl-lysine at residue 9 of human histone H3.

Specificity :

Recognizes monomethylated histone H3 (Lys9), Mr ~17 kDa.

Package Size :

100 µg

Uses :

Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.