monomethyl-Histone H3 (Lys27) Antibody | EMD Millipore 07-448 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

monomethyl-Histone H3 (Lys27) Antibody

catalog :

07-448

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

citations: 8

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse western blot; mouse; loading ...; fig 2e	Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, et al. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression. Proc Natl Acad Sci U S A. 2016;113:10370-5 pubmed publisher
ChIP-Seq; human; 1:250; loading ...; fig 4c western blot; human; 1:1000; loading ...; fig 4a	Su S, Li C, Lei P, Wang X, Zhao Q, Cai Y, et al. The EZH1-SUZ12 complex positively regulates the transcription of NF-?B target genes through interaction with UXT. J Cell Sci. 2016;129:2343-53 pubmed publisher
	Tan L, Zhang C, Hou X, Shao C, Lu Y, Zhou J, et al. The PEAT protein complexes are required for histone deacetylation and heterochromatin silencing. EMBO J. 2018;37: pubmed publisher
	Lee C, Yu J, Kumar S, Jin Y, LeRoy G, Bhanu N, et al. Allosteric Activation Dictates PRC2 Activity Independent of Its Recruitment to Chromatin. Mol Cell. 2018;70:422-434.e6 pubmed publisher
	Lee C, Holder M, Grau D, Saldaña Meyer R, Yu J, Ganai R, et al. Distinct Stimulatory Mechanisms Regulate the Catalytic Activity of Polycomb Repressive Complex 2. Mol Cell. 2018;70:435-448.e5 pubmed publisher
	Vergara Z, Sequeira Mendes J, Morata J, Peiro R, Henaff E, Costas C, et al. Retrotransposons are specified as DNA replication origins in the gene-poor regions of Arabidopsis heterochromatin. Nucleic Acids Res. 2017;45:8358-8368 pubmed publisher
	Yang Y, La H, Tang K, Miki D, Yang L, Wang B, et al. SAC3B, a central component of the mRNA export complex TREX-2, is required for prevention of epigenetic gene silencing in Arabidopsis. Nucleic Acids Res. 2017;45:181-197 pubmed publisher
	Baker T, Nerle S, Pritchard J, Zhao B, Rivera V, Garner A, et al. Acquisition of a single EZH2 D1 domain mutation confers acquired resistance to EZH2-targeted inhibitors. Oncotarget. 2015;6:32646-55 pubmed publisher

product information

Catalog Number :

07-448

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-monomethyl-Histone H3 (Lys27) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

monomethyl-Histone H3 (Lys27)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-monomethyl-Histone H3 (Lys27) Antibody

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K27me1;Histone H3 (mono methyl K27);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Specificity :

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Package Size :

200 µg

Uses :

Dot Blot;Immunocytochemistry;Western Blotting;ChIP-seq

Storage :

Stable for 1 year at 2-8°C from date of receipt