trimethyl-Histone H3 (Lys9) Antibody | EMD Millipore 07-442 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H3 (Lys9) Antibody

catalog :

07-442

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:500; loading ...; fig 4a	Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, et al. Identification of the elementary structural units of the DNA damage response. Nat Commun. 2017;8:15760 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 4c, 5e	Luo D, de Morrée A, Boutet S, Quach N, Natu V, Rustagi A, et al. Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c. Proc Natl Acad Sci U S A. 2017;114:E3071-E3080 pubmed publisher
western blot; human; loading ...; fig 4a	Jin X, Wu N, Dai J, Li Q, Xiao X. TXNIP mediates the differential responses of A549 cells to sodium butyrate and sodium 4-phenylbutyrate treatment. Cancer Med. 2017;6:424-438 pubmed publisher
immunocytochemistry; mouse; fig 2 immunohistochemistry; human; fig 4 western blot; human; fig 4	Mansuroglu Z, Benhelli Mokrani H, Marcato V, Sultan A, Violet M, Chauderlier A, et al. Loss of Tau protein affects the structure, transcription and repair of neuronal pericentromeric heterochromatin. Sci Rep. 2016;6:33047 pubmed publisher
western blot; human; fig 2	Onyango D, Howard S, Neherin K, Yanez D, Stark J. Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res. 2016;44:5702-16 pubmed publisher
western blot; human; fig 1	Shearstone J, Golonzhka O, Chonkar A, Tamang D, Van Duzer J, Jones S, et al. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS ONE. 2016;11:e0153767 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 10 immunocytochemistry; human; 1:500; fig 10	Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, LlÃ¨res D, et al. The cell proliferation antigen Ki-67 organises heterochromatin. elife. 2016;5:e13722 pubmed publisher
immunocytochemistry; human; fig s2	Boulding T, Wu F, McCuaig R, Dunn J, Sutton C, Hardy K, et al. Differential Roles for DUSP Family Members in Epithelial-to-Mesenchymal Transition and Cancer Stem Cell Regulation in Breast Cancer. PLoS ONE. 2016;11:e0148065 pubmed publisher
immunocytochemistry; mouse; 1:200; fig 2	Ancelin K, Syx L, Borensztein M, Ranisavljevic N, Vassilev I, Briseño Roa L, et al. Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation. elife. 2016;5: pubmed publisher
chromatin immunoprecipitation; Xenopus laevis; fig s2	Tamaoki K, Okada R, Ishihara A, Shiojiri N, Mochizuki K, Goda T, et al. Morphological, biochemical, transcriptional and epigenetic responses to fasting and refeeding in intestine of Xenopus laevis. Cell Biosci. 2016;6:2 pubmed publisher
chromatin immunoprecipitation; human; fig 2 western blot; human; 1:2000; fig 7	MartÃnez Iglesias O, Alonso Merino E, GÃ³mez Rey S, Velasco MartÃn J, MartÃn Orozco R, Luengo E, et al. Autoregulatory loop of nuclear corepressor 1 expression controls invasion, tumor growth, and metastasis. Proc Natl Acad Sci U S A. 2016;113:E328-37 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 1	Cloutier J, Mahadevaiah S, Elinati E, TÃ³th A, Turner J. Mammalian meiotic silencing exhibits sexually dimorphic features. Chromosoma. 2016;125:215-26 pubmed publisher
	Peter C, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun. 2019;10:4112 pubmed publisher
	Capitano M, Mor Vaknin N, Saha A, Cooper S, Legendre M, Guo H, et al. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling. J Clin Invest. 2019;129:2555-2570 pubmed publisher
	Dion C, Roche S, Laberthonnière C, Broucqsault N, Mariot V, Xue S, et al. SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite. Nucleic Acids Res. 2019;47:2822-2839 pubmed publisher
	Wu Y, Li X, Yu J, Bjorkholm M, Xu D. ASF1a inhibition induces p53-dependent growth arrest and senescence of cancer cells. Cell Death Dis. 2019;10:76 pubmed publisher
	Kallimasioti Pazi E, Thelakkad Chathoth K, Taylor G, Meynert A, Ballinger T, Kelder M, et al. Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair. PLoS Biol. 2018;16:e2005595 pubmed publisher
	Mikaeili H, Sandi M, Bayot A, Al Mahdawi S, Pook M. FAST-1 antisense RNA epigenetically alters FXN expression. Sci Rep. 2018;8:17217 pubmed publisher
	Hirota T, Blakeley P, Sangrithi M, Mahadevaiah S, Encheva V, Snijders A, et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell. 2018;47:645-659.e6 pubmed publisher
	Moon S, Im S, Kim N, Youn H, Park U, Kim J, et al. Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis. Cell Death Dis. 2018;9:1118 pubmed publisher
	Bayo J, Tran T, Wang L, Peña Llopis S, Das A, Martinez E. Jumonji Inhibitors Overcome Radioresistance in Cancer through Changes in H3K4 Methylation at Double-Strand Breaks. Cell Rep. 2018;25:1040-1050.e5 pubmed publisher
	Han F, Miyagawa Y, Verlengia G, Ingusci S, Soukupova M, Simonato M, et al. Cellular Antisilencing Elements Support Transgene Expression from Herpes Simplex Virus Vectors in the Absence of Immediate Early Gene Expression. J Virol. 2018;92: pubmed publisher
	Lee C, Yu J, Kumar S, Jin Y, LeRoy G, Bhanu N, et al. Allosteric Activation Dictates PRC2 Activity Independent of Its Recruitment to Chromatin. Mol Cell. 2018;70:422-434.e6 pubmed publisher
	Li Q, Tjong H, Li X, Gong K, Zhou X, Chiolo I, et al. The three-dimensional genome organization of Drosophila melanogaster through data integration. Genome Biol. 2017;18:145 pubmed publisher
	Choi J, Yoon S, Kim S, Ahn Jo S. KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 2017;7:45005 pubmed publisher
	Marion R, Lopez de Silanes I, Mosteiro L, Gamache B, Abad M, Guerra C, et al. Common Telomere Changes during In Vivo Reprogramming and Early Stages of Tumorigenesis. Stem Cell Reports. 2017;8:460-475 pubmed publisher
	Sohi G, Marchand K, Revesz A, Arany E, Hardy D. Maternal protein restriction elevates cholesterol in adult rat offspring due to repressive changes in histone modifications at the cholesterol 7alpha-hydroxylase promoter. Mol Endocrinol. 2011;25:785-98 pubmed publisher
	Liu Y, Taverna S, Muratore T, Shabanowitz J, Hunt D, Allis C. RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena. Genes Dev. 2007;21:1530-45 pubmed

product information

Catalog Number :

07-442

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H3 (Lys9) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

trimethyl-Histone H3 (Lys9)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-trimethyl-Histone H3 (Lys9) Antibody

Cross Reactivity :

Human;Mouse;Rat;Chicken

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K9me3;Histone H3 (tri methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, 2X-branched synthetic peptide containing the sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3.

Specificity :

Trimethyl-histone H3 (Lys9). Broad species cross-reactivity expected.

Package Size :

100 µg

Uses :

Dot Blot;Immunocytochemistry;Multiplexing;Peptide Inhibition Assay;Western Blotting;ChIP-seq

Storage :

1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.