dimethyl-Histone H3 (Lys9) Antibody | EMD Millipore 07-441 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Lys9) Antibody

catalog :

07-441

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:250; loading ...; fig e6h	Borsos M, Perricone S, Schauer T, Pontabry J, de Luca K, de Vries S, et al. Genome-lamina interactions are established de novo in the early mouse embryo. Nature. 2019;: pubmed publisher
immunoprecipitation; human; loading ...; fig s1d	Chae Y, Kim J, Park J, Kim K, Oh H, Lee K, et al. FOXO1 degradation via G9a-mediated methylation promotes cell proliferation in colon cancer. Nucleic Acids Res. 2019;47:1692-1705 pubmed publisher
immunohistochemistry; human; fig s6	Sareddy G, Viswanadhapalli S, Surapaneni P, Suzuki T, Brenner A, Vadlamudi R. Novel KDM1A inhibitors induce differentiation and apoptosis of glioma stem cells via unfolded protein response pathway. Oncogene. 2017;36:2423-2434 pubmed publisher
chromatin immunoprecipitation; human; fig 6	Kang M, Park K, Yang J, Lee C, Oh S, Yun J, et al. miR-6734 Up-Regulates p21 Gene Expression and Induces Cell Cycle Arrest and Apoptosis in Colon Cancer Cells. PLoS ONE. 2016;11:e0160961 pubmed publisher
western blot; human; fig 1	Jayaram H, Hoelper D, Jain S, Cantone N, Lundgren S, Poy F, et al. S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3. Proc Natl Acad Sci U S A. 2016;113:6182-7 pubmed publisher
chromatin immunoprecipitation; Xenopus laevis; fig s2	Tamaoki K, Okada R, Ishihara A, Shiojiri N, Mochizuki K, Goda T, et al. Morphological, biochemical, transcriptional and epigenetic responses to fasting and refeeding in intestine of Xenopus laevis. Cell Biosci. 2016;6:2 pubmed publisher
immunocytochemistry; mouse; 1:400; fig s3	Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill P, D Souza Z, et al. De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat Cell Biol. 2016;18:225-233 pubmed publisher
immunohistochemistry; mouse; 1:500	Murakami K, GÃ¼nesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher
	Fan L, Xu S, Zhang F, Cui X, Fazli L, Gleave M, et al. Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells. Cell Death Dis. 2020;11:214 pubmed publisher
	Peter C, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun. 2019;10:4112 pubmed publisher
	Fan L, Zhang F, Xu S, Cui X, Hussain A, Fazli L, et al. Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells. Proc Natl Acad Sci U S A. 2018;115:E4584-E4593 pubmed publisher
	Gu W, Mochizuki K, Otsuka K, Hamada R, Takehara A, Matsui Y. Dnd1-mediated epigenetic control of teratoma formation in mouse. Biol Open. 2018;7: pubmed publisher
	Zhao D, Hamilton J, Vaillancourt B, Zhang W, Eizenga G, Cui Y, et al. The unique epigenetic features of Pack-MULEs and their impact on chromosomal base composition and expression spectrum. Nucleic Acids Res. 2018;46:2380-2397 pubmed publisher
	Anreiter I, Kramer J, Sokolowski M. Epigenetic mechanisms modulate differences in Drosophila foraging behavior. Proc Natl Acad Sci U S A. 2017;114:12518-12523 pubmed publisher
	Carnesecchi J, Forcet C, Zhang L, Tribollet V, Barenton B, Boudra R, et al. ERR? induces H3K9 demethylation by LSD1 to promote cell invasion. Proc Natl Acad Sci U S A. 2017;114:3909-3914 pubmed publisher
	Tsukamoto D, Ito M, Takamatsu N. HNF-4 participates in the hibernation-associated transcriptional regulation of the chipmunk hibernation-related protein gene. Sci Rep. 2017;7:44279 pubmed publisher

product information

Catalog Number :

07-441

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Lys9) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Lys9)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Lys9) Antibody

Cross Reactivity :

Human;Mouse;Rat;Chicken;Yeast ( S. pombe )

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K9me2;Histone H3 (di methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, 2X-branched synthetic peptide containing the sequence . Rme2KSTG. , in which me2K corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Specificity :

Histone H3 dimethylated on lysine 9.

Package Size :

100 µg

Uses :

Dot Blot;Immunocytochemistry;Peptide Inhibition Assay;Western Blotting

Storage :

1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.