BRCA1 Antibody | EMD Millipore 07-434 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BRCA1 Antibody

catalog :

07-434

quantity :

100 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 1a western blot; human; loading ...; fig 3a	Batenburg N, Walker J, Coulombe Y, Sherker A, Masson J, Zhu X. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection. Nucleic Acids Res. 2019;47:10678-10692 pubmed publisher
western blot; human; loading ...; fig 1a	Wang H, Xiang D, Liu B, He A, Randle H, Zhang K, et al. Inadequate DNA Damage Repair Promotes Mammary Transdifferentiation, Leading to BRCA1 Breast Cancer. Cell. 2019;178:135-151.e19 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4b	Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher
	Simhadri S, Vincelli G, Huo Y, Misenko S, Foo T, Ahlskog J, et al. PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response. Oncogene. 2019;38:1585-1596 pubmed publisher
	Foo T, Tischkowitz M, Simhadri S, Boshari T, Zayed N, Burke K, et al. Compromised BRCA1-PALB2 interaction is associated with breast cancer risk. Oncogene. 2017;36:4161-4170 pubmed publisher

product information

Catalog Number :

07-434

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BRCA1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P38398

Host Name :

Rabbit

Antigen :

BRCA1

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-BRCA1 Antibody

Cross Reactivity :

Human

Background :

Two major susceptibility genes for breast cancer, BRCA1 and BRCA2, have been identified. Both genes are considered to be tumor-suppressor genes. Mutations within BRCA1 and BRCA2 are responsible for most familial breast cancer cases. Functional analyses of the BRCA1 and BRCA2 gene products have established their dual participation in transcription regulation and DNA damage repair. BRCA1 affects cell cycle regulation and loss of BRCA1 function due to decreased expression leads to cell cycle arrest, through p53 and p21 genes. In vivo, BRCA1 and BRCA2 are expressed at maximal levels in rapidly proliferating cells. This feature is consistent with in vitro observations that BRCA1 and BRCA2 are expressed in a cell cycle-dependent manner. During mammary gland development, the expression of BRCA1 and BRCA2 is induced in rapidly proliferating cellular compartments.

Immunogen :

GST-tagged fusion protein corresponding to amino acids 1301-1863 of human BRCA1.

Specificity :

Recognizes human BRCA1.

Package Size :

100 μL

Uses :

Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.