K+/Cl- Cotransporter (KCC2) Antibody | EMD Millipore 07-432 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

K+/Cl- Cotransporter (KCC2) Antibody

catalog :

07-432

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig 3a	Lorenzo L, Godin A, Ferrini F, Bachand K, Plasencia Fernandez I, Labrecque S, et al. Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain. Nat Commun. 2020;11:869 pubmed publisher
western blot; rat; loading ...; fig 3b1	Lee M, Liu Y, Chen C, Lu C, Lu S, Huang T, et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol. 2020;219: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1b	Richner M, Pallesen L, Ulrichsen M, Poulsen E, Holm T, Login H, et al. Sortilin gates neurotensin and BDNF signaling to control peripheral neuropathic pain. Sci Adv. 2019;5:eaav9946 pubmed publisher
immunoprecipitation; rat; loading ...; fig 1C immunocytochemistry; rat; loading ...; fig 1E western blot; rat; loading ...; fig 1B	Wright R, Newey S, Ilie A, Wefelmeyer W, Raimondo J, Ginham R, et al. Neuronal Chloride Regulation via KCC2 Is Modulated through a GABAB Receptor Protein Complex. J Neurosci. 2017;37:5447-5462 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 2-s1	Forsberg D, Horn Z, Tserga E, Smedler E, Silberberg G, Shvarev Y, et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. elife. 2016;5: pubmed publisher
immunohistochemistry; rat; 1:1000; loading ...; fig 4g	Wagner F, Bernard R, Derst C, French L, Veh R. Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance. Brain Struct Funct. 2016;221:4663-4689 pubmed
immunohistochemistry; rat; 1:200; fig 4 western blot; rat; 1:1000; fig 1	Awad P, Sanon N, Chattopadhyaya B, CarriÃ§o J, Ouardouz M, GagnÃ© J, et al. Reducing premature KCC2 expression rescues seizure susceptibility and spine morphology in atypical febrile seizures. Neurobiol Dis. 2016;91:10-20 pubmed publisher
	Wan L, Ren L, Chen L, Wang G, Liu X, Wang B, et al. M-Calpain Activation Facilitates Seizure Induced KCC2 Down Regulation. Front Mol Neurosci. 2018;11:287 pubmed publisher
	Kelley M, Cardarelli R, Smalley J, Ollerhead T, Andrew P, Brandon N, et al. Locally Reducing KCC2 Activity in the Hippocampus is Sufficient to Induce Temporal Lobe Epilepsy. EBioMedicine. 2018;32:62-71 pubmed publisher
	Heubl M, Zhang J, Pressey J, Al Awabdh S, Renner M, Gomez Castro F, et al. GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase. Nat Commun. 2017;8:1776 pubmed publisher
	Ferando I, Faas G, Mody I. Diminished KCC2 confounds synapse specificity of LTP during senescence. Nat Neurosci. 2016;19:1197-200 pubmed publisher

product information

Catalog Number :

07-432

Subcategory :

Neuroscience

Product Name :

Anti-K+/Cl- Cotransporter (KCC2) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q9H2X9

Host Name :

Rabbit

Antigen :

K+/Cl- Cotransporter (KCC2)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-K+/Cl- Cotransporter (KCC2) Antibody

Cross Reactivity :

Rat;Canine

Background :

KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

ALT Names :

Electroneutral potassium-chloride cotransporter 2;Erythroid K-Cl cotransporter 2;Neuronal K-Cl cotransporter;electroneural potassium-chloride cotransporter 2;solute carrier family 12 (potassium-chloride transporter), member 5

Immunogen :

N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).

Specificity :

Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band 200 kDa was also detected.

Package Size :

100 µg

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.