phospho-eNOS (Ser1177) Antibody | EMD Millipore 07-428 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-eNOS (Ser1177) Antibody

catalog :

07-428

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dogs, bovine

application :

western blot

citations: 2

Reference
Burke S, Zsengellér Z, Khankin E, Lo A, Rajakumar A, Dupont J, et al. Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia. J Clin Invest. 2016;126:2561-74 pubmed publisher
Tran J, Magenau A, Rodriguez M, Rentero C, Royo T, Enrich C, et al. Activation of Endothelial Nitric Oxide (eNOS) Occurs through Different Membrane Domains in Endothelial Cells. PLoS ONE. 2016;11:e0151556 pubmed publisher

product information

Catalog Number :

07-428

Subcategory :

Neuroscience

Product Name :

Anti-phospho-eNOS (Ser1177) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P29474

Host Name :

Rabbit

Antigen :

phospho-eNOS (Ser1177)

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-phospho-eNOS (Ser1177) Antibody

Cross Reactivity :

Human;Mouse;Rat;Bovine;Canine

Background :

NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.

ALT Names :

Constitutive NOS;Endothelial NOS;Endothelial NOS;Endothelial NOS;endothelial nitric oxide synthase;nitric oxide synthase 3 (endothelial cell)

Immunogen :

KLH-conjugated, synthetic peptide (C-RIRTQ[pS]FSLQER) corresponding to amino acids 1172-1183 of human eNOS (endothelial nitric oxide synthase) with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat, mouse, bovine, pig, and dog.

Specificity :

Recognizes phosphorylated eNOS, Mr 132 kDa. Additional unknown bands at 50 and 64 kDa were competed by the immunizing peptide.

Package Size :

200 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.