Akt1/PKBα Antibody | EMD Millipore 07-416 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Akt1/PKBα Antibody

catalog :

07-416

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	Stepanenko A, Andreieva S, Korets K, Mykytenko D, Baklaushev V, Huleyuk N, et al. Temozolomide promotes genomic and phenotypic changes in glioblastoma cells. Cancer Cell Int. 2016;16:36 pubmed publisher
western blot; mouse; fig s3a	Llanos S, GarcÃa Pedrero J, Morgado Palacin L, Rodrigo J, Serrano M. Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers. Nat Commun. 2016;7:10438 pubmed publisher

product information

Catalog Number :

07-416

Subcategory :

Cell Signaling

Product Name :

Anti-Akt1/PKBα Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P31749

Host Name :

Rabbit

Antigen :

Akt1/PKBα

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Akt1/PKBα Antibody

Cross Reactivity :

Human;Rat;Mouse

Background :

Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3, which is inactivated by this phosphorylation, the FoxO family of transcription factors, PRAS40, AS160.

ALT Names :

Protein kinase B;RAC-alpha serine/threonine-protein kinase;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

15 residue synthetic peptide (C-RPHFPQFS YSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1, with an N-terminal cysteine added for conjugation purposes. The Akt1 sequence is identical to mouse Akt1 (15/15 amino acids) and strongly conserved in bovine Akt1 (14/15 amino acids) and human Akt1 (13/15 amino acids). Human Akt2 shares 11/15 amino acids with the peptide immunogen sequence.

Specificity :

Recognizes Akt1/PKBα, MW ~60 kDa.

Package Size :

100 μg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.