Smad2/3 Antibody | EMD Millipore 07-408 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Smad2/3 Antibody

catalog :

07-408

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
	Dagher Z, Gerhardinger C, Vaz J, Goodridge M, Tecilazich F, Lorenzi M. The Increased Transforming Growth Factor-Î² Signaling Induced by Diabetes Protects Retinal Vessels. Am J Pathol. 2017;187:627-638 pubmed publisher

product information

Catalog Number :

07-408

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Smad2/3 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q15796

Host Name :

Rabbit

Antigen :

Smad2/3

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-Smad2/3 Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

ALT Names :

MAD, mothers against decapentaplegic homolog 2 (Drosophila);Mad protein homolog;Mad, mothers against decapentaplegic homolog 2;Mad-related protein 2;Mothers against DPP homolog 2;SMAD 2;SMAD family member 2;SMAD, mothers against DPP homolog 2;SMAD, mothers against DPP homolog 2 (Drosophila);Sma- and Mad-related protein 2;mother against DPP homolog 2

Immunogen :

Fusion protein corresponding to amino acids 186-273 of human Smad2.

Specificity :

Recognizes human Smad2 Mr 55-60 kDa and Smad3 Mr 50 kDa as determined by using Smad2 and Smad3 transfected Cos cells.

Package Size :

200 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.