PRMT5 Antibody | EMD Millipore 07-405 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PRMT5 Antibody

catalog :

07-405

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s5 western blot; human; 1:5000; loading ...; fig 5b	Demetriadou C, Pavlou D, Mpekris F, Achilleos C, Stylianopoulos T, Zaravinos A, et al. NAA40 contributes to colorectal cancer growth by controlling PRMT5 expression. Cell Death Dis. 2019;10:236 pubmed publisher
western blot; human; loading ...; fig 1d	Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 4 chromatin immunoprecipitation; human; loading ...; fig 3a,3b immunoprecipitation; human; loading ...; fig 3a,3b western blot; human; loading ...; fig 1e,2d immunohistochemistry - paraffin section; mouse; loading ...; fig 5	Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
immunocytochemistry; human; loading ...; fig 1g western blot; human; 1:5000; loading ...; fig s1c	Chen H, Lorton B, Gupta V, Shechter D. A TGFβ-PRMT5-MEP50 axis regulates cancer cell invasion through histone H3 and H4 arginine methylation coupled transcriptional activation and repression. Oncogene. 2017;36:373-386 pubmed publisher
western blot; human; 1:1000; fig 1a	Harris D, Chandrasekharan U, Bandyopadhyay S, Willard B, DiCorleto P. PRMT5-Mediated Methylation of NF-ÎºB p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-Î± and IFN-Î³ Costimulation. PLoS ONE. 2016;11:e0148905 pubmed publisher
	Rehman I, Basu S, Das S, Bhattacharjee S, Ghosh A, Pommier Y, et al. PRMT5-mediated arginine methylation of TDP1 for the repair of topoisomerase I covalent complexes. Nucleic Acids Res. 2018;46:5601-5617 pubmed publisher
	Lammirato A, Patsch K, Feiereisen F, Maly K, Nofziger C, Paulmichl M, et al. TIS7 induces transcriptional cascade of methylosome components required for muscle differentiation. BMC Biol. 2016;14:95 pubmed
	Eberhardt A, Hansen J, Koster J, Lotta L, Wang S, Livingstone E, et al. Protein arginine methyltransferase 1 is a novel regulator of MYCN in neuroblastoma. Oncotarget. 2016;7:63629-63639 pubmed publisher
	Patel S, Bhumbra S, Paknikar R, Dressler G. Epigenetic mechanisms of Groucho/Grg/TLE mediated transcriptional repression. Mol Cell. 2012;45:185-95 pubmed publisher

product information

Catalog Number :

07-405

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PRMT5 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O14744

Host Name :

Rabbit

Antigen :

PRMT5

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-PRMT5 Antibody

Cross Reactivity :

Human;Mouse

Background :

Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.

ALT Names :

72 kDa ICln-binding protein;HMT1 hnRNP methyltransferase-like 5;Jak-binding protein 1;SKB1 homolog;SKB1 homolog (S. pombe);Shk1 kinase-binding protein 1 homolog;protein arginine methyltransferase 5;skb1 (S. pombe) homolog

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.

Specificity :

PRMT5, Mr ~70 kDa

Package Size :

200 µg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.