acetyl-Histone H3 (Lys14) Antibody | EMD Millipore 07-353 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

acetyl-Histone H3 (Lys14) Antibody

catalog :

07-353

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

fission yeast, human, mouse, brewer's yeast

application :

western blot, chromatin immunoprecipitation

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig 2c	Wang Z, Millard C, Lin C, Gurnett J, Wu M, Lee K, et al. Diverse nucleosome site-selectivity among histone deacetylase complexes. elife. 2020;9: pubmed publisher
western blot; brewer's yeast; 1:2000; loading ...; fig 3b	Bennett S, Cobos S, Meykler M, Fallah M, Rana N, Chen K, et al. Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J Vis Exp. 2019;: pubmed publisher
western blot; brewer's yeast; 1:5000; loading ...; fig s1e	Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 7a	Schauwecker S, Kim J, Licht J, Clevenger C. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. J Biol Chem. 2017;292:2237-2254 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig s4d	Wang D, Kon N, Lasso G, Jiang L, Leng W, Zhu W, et al. Acetylation-regulated interaction between p53 and SET reveals a widespread regulatory mode. Nature. 2016;538:118-122 pubmed publisher
western blot; mouse; loading ...; fig 8a	You L, Li L, Zou J, Yan K, Belle J, Nijnik A, et al. BRPF1 is essential for development of fetal hematopoietic stem cells. J Clin Invest. 2016;126:3247-62 pubmed publisher
chromatin immunoprecipitation; fission yeast; fig 4e	Materne P, Vazquez E, Sanchez M, Yague Sanz C, Anandhakumar J, Migeot V, et al. Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus. elife. 2016;5: pubmed publisher
western blot; mouse; fig 3g	Hagelkruys A, Mattes K, Moos V, Rennmayr M, Ringbauer M, Sawicka A, et al. Essential Nonredundant Function of the Catalytic Activity of Histone Deacetylase 2 in Mouse Development. Mol Cell Biol. 2016;36:462-74 pubmed publisher
	Bao K, Shan C, Moresco J, Yates J, Jia S. Anti-silencing factor Epe1 associates with SAGA to regulate transcription within heterochromatin. Genes Dev. 2019;33:116-126 pubmed publisher
	Watts B, Wittmann S, Wery M, Gautier C, Kus K, Birot A, et al. Histone deacetylation promotes transcriptional silencing at facultative heterochromatin. Nucleic Acids Res. 2018;46:5426-5440 pubmed publisher
	Wang L, Zhang F, Rode S, Chin K, Ko E, Kim J, et al. Ethylene induces combinatorial effects of histone H3 acetylation in gene expression in Arabidopsis. BMC Genomics. 2017;18:538 pubmed publisher
	Jih G, Iglesias N, Currie M, Bhanu N, Paulo J, Gygi S, et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature. 2017;547:463-467 pubmed publisher
	Choi J, Yoon S, Kim S, Ahn Jo S. KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 2017;7:45005 pubmed publisher
	Tsukamoto D, Ito M, Takamatsu N. HNF-4 participates in the hibernation-associated transcriptional regulation of the chipmunk hibernation-related protein gene. Sci Rep. 2017;7:44279 pubmed publisher
	Porter E, Dykhuizen E. Individual Bromodomains of Polybromo-1 Contribute to Chromatin Association and Tumor Suppression in Clear Cell Renal Carcinoma. J Biol Chem. 2017;292:2601-2610 pubmed publisher
	Castonguay E, White S, Kagansky A, St Cyr D, Castillo A, Brugger C, et al. Panspecies small-molecule disruptors of heterochromatin-mediated transcriptional gene silencing. Mol Cell Biol. 2015;35:662-74 pubmed publisher

product information

Catalog Number :

07-353

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-acetyl-Histone H3 (Lys14) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

acetyl-Histone H3 (Lys14)

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-acetyl-Histone H3 (Lys14) Antibody

Cross Reactivity :

Human;Yeast ( S. cerevisiae )

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

ALT Names :

H3K14Ac;Histone H3 (acetyl K14);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to amino acids 9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.

Specificity :

Recognizes acetyl-histone H3 (Lys14), Mr ~17 kDa. An additional unknown protein was detected at Mr ~33 kDa.

Package Size :

100 µL

Uses :

Western Blotting;ChIP-seq;Dot Blot;Multiplexing;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.